Advanced Solutions
for Advanced Pathology
Cytokeratin 20 (Ks20.8)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45139 IMPATH Cytokeratin 20 RTU M (Ks20.8)
50 Tests
44269 Cytokeratin 20 RTU M (Ks20.8)
7 ml Ready To Use
44559 Cytokeratin 20 0,1 M (Ks20.8)
100 µl liquid Concentrated
44560 Cytokeratin 20 1 M (Ks20.8)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Colon Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
This antibody reacts primarily with gastric and intestinal epithelium, urothelium, and Merkel cells. Anti-cytokeratin 20 is useful in the differentiation
of specific types of simple epithelial cells of the urinary tract as well as normal and malignantly transformed epithelia. Studies have identified
the presence of cytokeratin 20 in adenocarcinomas of the colon, stomach, pancreas, and biliary system. Additionally, mucinous ovarian tumors,
transitional cell, and Merkel cell carcinomas have shown reactivity. In contrast, squamous cell carcinomas and adenocarcinomas of the breast,
lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas are non-reactive.
Bladder: Dysplasia vs. Reactive
CK 20
p53
CD44
Ki-67
Carcinoma-in-situ
+
+
-
+
Reactive Atypia
-
-
+
+
Normal Urothelium
+
-
+(basal layer)
-
Carcinomas
CK 20
CK
Cocktail
CK 7
CD10 β-Catenin CK 5
p63
CEA CDX-2
Villin
Lung Adenocarcinoma
-
+
+
-
-
-
+
-
-
Colorectal Adenocarcinoma
+
+
-
+
+
-
-
+
+
+
Transitional Cell Carcinoma
+
+
+
+
-
+
+
-
-
-
Squamous Cell Carcinoma
-
+
-
-
-
+
+
-
-
-
Cutaneous Neoplasm
CK 20
CD10
Androgen
Receptor
CD34
Ber-EP4
BCL2
CK 19
Basal Cell Carcinoma
-
+
+
-
+
+
+
Trichoepithelioma
+
-
-
+
+
+
+
Merkel Cell Carcinoma
+
-
-
-
+
+
+
Microcystic Adnexal
Carcinoma
-
+/-
-
-
-/+
+
Sebaceous Carcinoma
-
+/-
+
-
+
+/-
-
Sebaceous Adenoma
-
-
+
-
+
+
-
Reference
1. Moll R, et al. Histochem Cell Biol. 2008; 129:705-33.
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