Advanced Solutions
for Advanced Pathology
C3d (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45295 IMPATH C3d RTU R (Poly)
50 Tests
44209 C3d RTU R (Poly)
7 ml Ready To Use
44443 C3d 0,1 R (Poly)
100 µl liquid Concentrated
44444 C3d 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Acute rejected kidney transplant
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative
complement activation pathways. C3d deposition in the renal transplant PTCs (peritubular capillaries) is indicative of AR (acute rejection) with
subsequent high probability of graft loss. Anti-C3d, combined with anti-C4d, can be utilized as a tool for diagnosis of AR that may serve to
warrant prompt and aggressive anti-rejection treatment. Pfaltz et al. have shown that anti-C3d labeled the epidermal basement membrane in
97% (31/32) cases of bullous pemphigoid (BP), with none of the normal controls demonstrating such findings. In the same study 27% (3/11)
cases of pemphigus vulgaris (PV) demonstrated intercellular C3d deposition. Anti-C3d immunohistochemistry is a helpful adjunct in the diagnosis
of BP (and perhaps PV), especially in the cases in which only formalin-fixed, paraffin-embedded tissue is available for analysis.
Reference
1. Bickerstaff A, et al. Am J Pathol. 2008 Aug; 173 (2):347-57.
2. Kuypers DR, et al. Transplantation. 2003 Jul 15; 76 (1):102-8.
3. Pfaltz K, et al. J Cutan Pathol. 2009 Oct 15 (Epub ahead of print).
4. Eggertsen G, et al. APMIS. 2001 Dec; 109(12):825-34.
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