Antibodies for
Immunohistochemistry
CD117, c-kit (YR145
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45273 IMPATH CD117 RTU R (YR145)
50 Tests
44218 CD117 RTU R (YR145)
7 ml Ready To Use
44460 CD117 0,1 R (YR145)
100 µl liquid Concentrated
44461 CD117 1 R (YR145)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Gastrointestinal stromal tumor,
Seminoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD117, c-kit, is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium,
and mast cells. This receptor is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas
from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma;
however, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, tumors of smooth
muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and
clot section.
GIST Mutation vs. Wild Type
CD117
DOG1
CD34
GIST, Kit Mutation
+
+
+
GIST, PDGFRA Mutation
-
+
-
GIST, Wild Type
+
+
+/-
Mastocytosis
CD117
Tryptase
CD25
CD163
CD2
Systemic Mastocytosis
+
+
+
-
+
Reactive Mast Cells
+
+
-
+
-
Germ Cell Tumors
CD117 Oct-4
AFP
CK
Cocktail
EMA Inhibin D2-40
CD30 Vimentin PLAP
Seminoma
+
+
-
-
-
-
+
-
+
+
Embryonal Carcinoma
-
+
-
+
-
-
-
+
-
+
Kidney: Renal Epithelial Tumors
CD117
RCC
CD10
S100A1
Vimentin
Ksp-cadherin Parvalbumin
Clear Cell RCC
-
+
+
+
+
-
-
Chromophobe RCC
-/+
-/+
-/+
-
-
+
+
Papillary RCC
+
+
+
+
+
-/+
-
Oncocytoma
+
-
+/-
+
-
+/-
+
Reference
1. Sircar K, et al. AM J Surg Pathol. 1999; 23:377-389.
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