Antibodies for
Immunohistochemistry
CD45RO (UCHL-1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45132 IMPATH CD45RO RTU M (UCHL-1)
50 Tests
44243 CD45RO RTU M (UCHL-1)
7 ml Ready To Use
44507 CD45RO 0,1 M (UCHL-1)
100 µl liquid Concentrated
44508 CD45RO 1 M (UCHL-1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous, Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP 2-step
Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD45RO (Pan T-cell) reacts with thymocytes and activated T-cells, but only on a subpopulation of resting T-cells. This antibody shows no
reactivity with B-cells making it a good marker for T-cell tumors. In addition, granulocytes and monocytes are also labeled with this antibody.
T-cell, Pan has been designated as CD45RO at The International Leukocyte Typing Workshop.
Lymphoma
CD45RO
CD20
CD45R
CD43
CD3
Mature B-cell
-
+
+
-
-
Mature T-cell
+
-
-
+
+
T-cell Lymphomas
CD45RO CD45
CD2
CD3
CD4
CD5
CD7
CD8
CD25
PD-1
Angioimmunoblastic
+
+
+
+
+
+
+
-
+
+
Lymphoblastic
+
+
+/-
+
+/-
+
+
+/-
+
-
Subcutaneous
Panniculitis-Like
+
+
+
+
-
+
+
+/-
-
-
NK
+
+
+
+
-
-
-/+
-
+
-
Cutaneous
-
+
+
+
+
-
+
-
-
-/+
Peripheral, NOS
+
+
+
+
+/-
+/-
+/-
-/+
+
-
Mycosis Fungoides
+
+
+
+
+
+
-
-
+
-
Reference
1. Hall PA, et al. J Clin Path. 1987; 40:151-156.
2. Smith SH, et al. Immunology. 1986; 58:63-70.
3. Cabecadas JM, et al. Histopathology. 1991.
4. Tworek JA, et al. Am J Clin Pathol. 1998 Nov; 110(5):582-9.
5. Falini B, et al. Hum Pathol. 1990 Jun; 21(6):624-9.
6. Koch AE, et al. J Clin Immunol. 1990 Jul; 109(4):192-9.
7. Ritter JH, et al. J Cutan Pathol. 1994 Dec; 21(6):481-93.
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