Antibodies for
Immunohistochemistry
CD63 (NKI/C3)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45136 IMPATH CD63 RTU M (NKI/C3)
50 Tests
44248 CD63 RTU M (NKI/C3)
7 ml Ready To Use
44517 CD63 0,1 M (NKI/C3)
100 µl liquid Concentrated
44518 CD63 1 M (NKI/C3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Melanoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
This antibody reacts with a 53 kDa protein which forms a part of the family of tetraspan moieties. The antigen was originally designated as
a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse
distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells, and melanoma. It has been
quite useful in identifying malignant melanoma. It has also been reported as useful in differentiating renal oncocytoma (apical and/or polar
staining pattern) from eosinophilic renal cell carcinoma (diffuse cytoplasmic staining pattern). CD63 is well expressed in tissue mast cells in the
lung, skin, and bone marrow. It can be overexpressed on the surface of neoplastic mast cells in aggressive systemic mastocytosis although it is
less expressed in indolent systemic mastocytosis.
Normal Melanocytes, Benign and Malignant Melanotic Neoplasia
CD63
S-100
SOX-10
HMB-45
MART-1 Tyrosinase
MiTF
Factor XIIIa
Adult Melanocytes
+
+
+
-
+
+
+
-
Junctional Nevus
-
+
+
+
+
+
+
-
Interdermal Nevus
-
+
+
-
+
+
+
-
Primary Melanoma
+
+
+
+
+
+
+
-
Metastatic Melanoma
+
+
+
+
+
+
+
-
Spindle Cell Melanoma
+
+
+
+
+
+
+
-
Angiomyolipoma
+
+
+
+
+
-
+
-
Adrenal Cortical
-
+
-
-
+
-
-
-
Intranodal Nevus Cells
-
+
+
-
+
+
+
-
Dermatofibroma
-
-
-
-
-
-
-
+
PEComa
CD63 HMB-45 MART-1 S-100 Tyrosinase SM Actin Calponin Caldesmon Desmin CD68
Angiomyolipoma
+
+
+
-
-
+
+
+
-
+
Lymphangiomyomatosis
+
+
+
-
-
+
+
+
-
-
Extrapulmonary Clear Cell
Tumor
+
+
+
+
-
+
-
-
-
-
Primary Cutaneous
PEComa
+
+
+
-
-
-
-
-
-
+/-
Pulmonary Clear Cell Sugar
Tumor
+
+
+
+/-
-
-
-
-
-
+/-
Reference
1. Azorsa DO, Hyman JA, Hildreth JE. Blood. 1991 Jul 15; 78(2):280-4.
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