Advanced Solutions
for Advanced Pathology
CD99 (EPR3097Y
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45192 IMPATH CD99 RTU R (EPR3097Y)
50 Tests
44255 CD99 RTU R (EPR3097Y)
7 ml Ready To Use
44531 CD99 0,1 R (EPR3097Y)
100 µl liquid Concentrated
44532 CD99 1 R (EPR3097Y)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Ewing’s Sarcoma, Pancreas
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD99/MIC-2 antigen is present on the cell membrane of Ewing’s sarcoma and primitive peripheral neuroectodermal tumors (PNET). It is
also present on some cells in bone marrow, lymph nodes, spleen, cortical thymocytes, granulosa cells of the ovary, CNS ependymal cells,
Sertoli cells of the testis, and endothelial cells. MIC-2 has also been identified in lymphoblastic lymphoma, rhabdomyosarcoma, mesenchymal
chondrosarcoma, and thymoma.
Soft Tissue Sarcoma
CD99
CK
Cocktail
S-100 MS Actin SM Actin CD34
TLE-1
CD56
TFE-3 Calretinin
Synovial Sarcoma
+
+
-
-
-
-
+
+
-
+/-
Clear Cell Sarcoma
-
-
+
-
-
-
-
-
-
-
PNET/ES
+
-/+
+
-
-
-
-
-
-
-
Desmoplastic Small Round
Cell
-
+
-
-
-
-
-
-
-
-
Mesenchymal
Chondrosarcoma
+
-
+/-
-
-
-/+
-
-
-
+
Alveolar Soft Part Sarcoma
-
-
-
+
+
-
-
-
+
-
PEComa
-
-
-
-
+
-
-
-
-
+
Skin: Spindle Cell Tumors
CD99
FLI-1 GLUT1 Factor VIII
HHV-8 CD10
CD34
D2-40 SM Actin MS Actin
Atypical Fibroxanthomas
+
-
-
-
-
+
-
-
+
+
Angiosarcoma
-
+
-
+
-
-
+
+/-
-
-
Glomus Tumor
-
-
-
-
-
-
+/-
-
+
+
Solitary Fibrous Tumor
+/-
-/+
-
-
-
-
+
-
-
-
Hemangioma
-
+
+
+
-
-
+
-
+
-
Kaposi's Sarcoma
-
+
-
+
+
-
+
+
+
-
Sex Cord Stromal Tumors
CD99
Calretinin
Inhibin
CK 7
EMA
Vimentin
MART-1
Granulosa Cell Tumors
+
+
+
-
-
+
+
Sertoli-Leydig Cell Tumors
-/+
+
+
+
-
+
+
Gonadoblastomas
+
+
+
-
-
+
-
Reference
1. Rettig WJ, et al. Lab Invest. 1992; 66:133.
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