Antibodies for
Immunohistochemistry
CD71 (MRQ-48)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45189 IMPATH CD71 RTU M (MRQ-48)
50 Tests
44251 CD71 RTU M (MRQ-48)
7 ml Ready To Use
44523 CD71 0,1 M (MRQ-48)
100 µl liquid Concentrated
44524 CD71 1 M (MRQ-48)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Bone Marrow
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The transferrin receptor (CD71) is most highly expressed on placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes,
endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors
through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase. The maturation of erythrocytes
results in loss of transferrin receptor expression, in concert with downregulation of the machinery for hemoglobin synthesis. The high level of
transferrin receptor within erythroid precursors makes anti-CD71 an excellent marker for evaluation of erythroid precursors within bone marrow
biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone
marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of
erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes
do not express CD71, which facilitates bone marrow analyses. Anti-CD71 is useful in identifying erythroid precursors with very little interference
from mature erythrocytes and also in the determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic
syndrome, although further studies are needed for making a definitive diagnosis of myelodysplastic syndrome.
Erythroid
CD71
Glycophorin A
Hemoglobin A
Spectrin
Erythroid Hyperplasia
+
+
+
+
Erythroid Hypoplasia
+
+
+
+
Acute Erythroid Leukemia
+
+
+
+
Extramedullary
Hematopoiesis
+
+
+
+
Mature Erythrocytes
-
+
+
+
Reference
1. Ponka P, et al. Int J Biochem Cell Biol. 1999; 31:1111-1137.
2. Sieff C, et al. Blood. 1982; 60:703-713.
3. Lesley J, et al. Cell Immunol. 1984; 83:14-25.
4. Nakahata T, et al. Leuk Lymphoma. 1994; 13:401-409.
5. Marsee DK, et al. Am J Clin Pathology. 2010; 13:429-435.
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