Advanced Solutions
for Advanced Pathology
CD74 (LN2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44252 CD74 RTU M (LN2)
7 ml Ready To Use
44525 CD74 0,1 M (LN2)
100 µl liquid Concentrated
44526 CD74 1 M (LN2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Lymph Node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
CD74, also known as the MHC class II associated invariant chain (II), is a type II transmembrane protein which binds to the peptide binding
groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.
CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of
or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 minutes after which it is internalized. CD74
is expressed primarily by antigen presenting cells such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage),
macrophages, and monocytes, and many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on
genetic splicing.
Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas but rarely T-cell lymphomas. It stains the cell membrane but
a paranuclear globular labeling is also noted. It is a useful addition to the lymphoma phenotyping panel when B5 or alcohol fixed tissue is used.
Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Myeloid Leukemia, Cutaneous Myeloid Sarcoma
CD74
MPO CD68 Factor VIII
CD61 BOB.1 Oct-2 Glyco-
phorin A Spectrin CD34
Acute, M0
+
-
-
-
-
-
-
-
-
+
Acute, M1&2
+
+
+
-
-
-
-
+
Acute Myelomonocytic, M4
+
+
+
-
-
-
+
-
-
+
Acute Monoblastic, M5
+
+
+
-
-
-
+
-
-
-/+
Chronic
+
+
-
-
-
+/-
-
-
-
-/+
Lymphoblastic Lymphomas, BCL vs. TCL
CD74
TdT
CD10
PAX-5
CD20
CD19
CD3
CD5
CD7
CD117
Lymphoblastic BCL
+
+
+/-
+
+/-
+
-
-
-
-
Lymphoblastic TCL
-
+
+
-
-
-
+
+/-
+
-
Reference
1. Chan JKC, et al. Histopathology. 1994; 25:517-536.
2. Kaddu S, et al. J Am Acad Dermatol. 1999; 40:966-978.
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