Advanced Solutions
for Advanced Pathology
EMA (E29)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45205 IMPATH EMA RTU M (E29)
50 Tests
44282 EMA RTU M (E29)
7 ml Ready To Use
44586 EMA 0,1 M (E29)
100 µl liquid Concentrated
44587 EMA 1 M (E29)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Breast, Skin
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-EMA is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary
epithelium, sweat glands, and squamous epithelium. Hepatocellular carcinoma, adrenal carcinoma, and embryonal carcinomas are consistently
EMA negative, so keratin positivity with negative EMA favors one of these tumors. Anti-EMA is frequently positive in meningioma, which can be
useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas.
Hodgkin vs. Non-Hodgkin Lymphomas
EMA CD79a CD15
CD30 Fascin Granzyme
B
BCL6
PU.1
MUM1 ALK-1
Hodgkin Lymphoma,
Classic
-
-
+
+
+
-
-
-
+
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
+
+
-
-
-
-
+
+
-/+
-
T-cell Rich LBCL
-
+
-
-
-
-
+
-
+
-
Anaplastic LCL
+
-
-
+
-
+
+/-
-
-
+
Skin: Adnexal Tumors
EMA
CK 7
CK 20
S-100
GCDFP-15
CD15
Merkel Cell Carcinoma
+
-
+
-
-
-
Sebaceous Tumor
-
+
-
-
-
+
Apocrine Tumor
+/-
+
-
-
+
+/-
Eccrine Tumor
+
+
-
+
-
-
Skin: Basal vs. Squamous Cell Carcinoma
EMA
CK Cocktail
Ep-CAM
BCL2
Basal Cell Carcinoma
-
+
+
+
Squamous Cell Carcinoma
+
+
-
-
Brain: CNS Tumors
EMA
INI-1
Neurofilament
S-100
CK Cocktail
Vimentin
Meningioma
+
+
-
-
-
+
Rhabdoid Tumors
+
-
+
+/-
+
+
Reference
1. Pincus GS, et al. Human Pathol. 1985; 16:929-940.
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