Antibodies for
Immunohistochemistry
Cytokeratin, LMW (AE1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45302 IMPATH Cytokeratin LMW RTU M (AE1)
50 Tests
44276 Cytokeratin, LMW RTU M (AE1)
7 ml Ready To Use
44573 Cytokeratin, LMW 0,1 M (AE1)
100 µl liquid Concentrated
44574 Cytokeratin, LMW 1 M (AE1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Prostate, Salivary gland
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin, low molecular weight (AE1) labels most acidic keratins; therefore, it is a broadly reactive antibody staining most epithelia and
their neoplasms. Members of the acidic and basic cytokeratin subfamilies are found together in pairs; each epithelium contains at least one
acidic and one basic keratin so this antibody can show the distribution of keratin containing cells in epithelia. This antibody has shown great
sensitivity and broad specificity for keratins under various conditions of fixation and staining. Anti-low molecular weight cytokeratin (AE1) is
particularly suited to distinguish poorly differentiated carcinomas from non-epithelial neoplasms. This marker stains both normal and neoplastic
cells of epithelial origin.
Carcinomas
CK, LMW CK 7
CK 20 CK, HMW CK 5
TTF-1 GCDFP-15 pCEA Hep-Par1 RCC
Hepatocellular Carcinoma
-
-
-
-
-
+
(cytoplasmic)
-
+
+
-
Renal Cell Carcinoma
+
-
-
-
-
-
-
-
-
+
Bladder Carcinoma
+
+
+
+
-
-
-
+
-
-
Salivary Gland Carcinoma
+
+
-
+
+
-
+
-
-
Thyroid Carcinoma
+
+
-
-
+
-
-
-
-
Spindle Cell Carcinoma
-
-
+
-
-
-
-
-
Breast Carcinoma
+
+
-
+
-
-
+
-
-
-
Lung Adenocarcinoma
+
+
-
+
-
+
-
+
-
-
Colorectal Adenocarcinoma
+
-
+
-
-
-
-
+
-
-
Prostate Adenocarcinoma
+
-
-
-
-
-
-
-
-
-
Transitional Cell Carcinoma
+
+
+
+
+
-
-
-
-
-
Ovarian Carcinoma
+
+
-
+
+
-
-
-
-
-
Pancreatic Carcinoma
+
+
-
+/-
-
-
-
+
-
-
Squamous Cell Carcinoma
+
-
-
+
+
-
-
-
-
-
Skin: Pagetoid Tumors
CK, LMW
CK, HMW
S-100
CEA
Vimentin
Melanoma
-
-
+
-
+
Paget’s Disease
+
-
-/+
+
-
Bowen’s Disease
+
+
-
-
-
Reference
1. Tyler CR. Arch Pathol Lab Med. 1978; 102:113.
108
