Antibodies for
Immunohistochemistry
E-cadherin (EP700Y
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45283 IMPATH E-cadherin RTU R (EP700Y)
50 Tests
44281 E-Cadherin RTU R (EP700Y)
7 ml Ready To Use
44584 E-Cadherin 0,1 R (EP700Y)
100 µl liquid Concentrated
44585 E-Cadherin 1 R (EP700Y)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Breast
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
E-cadherin is an adhesion protein that is expressed in cells of epithelial lineage. Anti-E-cadherin stains positively in glandular epithelium as well
as adenocarcinomas of the lung, G.I. tract, and ovary. Another application involves the differentiation of ductal (which stains positive) vs. lobular
cancer of the breast. It has also been shown to be positive in some thyroid carcinomas Loss of E-cadherin expression has been suggested as a
poor prognostic sign in breast carcinoma and non-small cell lung carcinoma.
Small Cell Carcinoma vs. Merkel Cell Carcinoma
E-cadherin
TTF-1
CEA
CK 20
Chromo-
granin A
Neuro-
filament
CD117
Synapto-
physin
Merkel Cell Carcinoma
+(nuclear)
-
-
+
+
+
+
+
Small Cell Carcinoma
-
+
-
-
-
-
+/-
+
Breast Carcinoma
E-cadherin
GCDFP-15
Mammaglobin
β-Catenin
CK, 34βE12
p120
Lobular
-
+
+
-
+
+(cytoplasmic)
Ductal
+
+
+
+(membranous)
-
+(membranous)
Pancreas
E-cadherin Synapto-
physin
Chromo-
granin A CD10 Gastrin CK 19 CA19-9 CD56 β-Catenin S100P
Neuroendocrine Tumor
-
+
+
-
+/-
+/-
+/-
+
+
-
Solid Pseudopapillary
Tumor
+(nuclear)
+
-
+
-
-
-
+
+
-
Ductal Carcinoma
+/-
-
-
+/-
-
+
+
-
+/-
+
Acinic Cell Carcinoma
+
-
-
+/-
-
+
-/+
-
+
-
Pancreatoblastoma
-
-
+
-
-
-
-
+
+
-
Normal Pancreas
-
+
+
-
-
-
-
-
+
-
Reference
1. Han AC, et al. Hum Pathol. 1997 Jun; 28/(6):641-5.
2. Simsir A, et al. Diagn Cytopathol. 1999 Mar; 20(3):125-30.
3. Han AC, et al. Cancer. 1999 Apr 25; 87(2):83-6.
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