Antibodies for
Immunohistochemistry
Ep-CAM/Epithelial Specific Antigen (Ber-EP4)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45648 IMPATH Ep-CAM RTU M (Ber-EP4) 50 Tests
45635 Ep-CAM RTU M (Ber-EP4)
7 ml Ready To Use
45614 Ep-CAM 0,1 M (Ber-EP4)
100 µl liquid Concentrated
45615 Ep-CAM 1 M (Ber-EP4)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Adenocarcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Ep-CAM consists of two glycoproteins, 34 and 39 kDa, sometimes designated as epithelial antigen, epithelial specific antigen, or epithelial
glycoprotein. In paraffin sections, the protein is detected with mouse anti-human antibodies like anti-Ber-EP4 and anti-MOC-31. The
glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous
epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells, and myoepithelial cells. In liver lesions like hepatitis and cirrhosis, the
hepatocytes frequently become anti-Ep-CAM positive. Normal mesothelial cells are anti-Ep-CAM negative, but may express focal reaction when
undergoing reactive changes. Ep-CAM is found in the large majority of adenocarcinomas of most sites (50%-100% in various studies) as well as
in neuroendocrine tumors, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain with anti-Ber-EP4 in about
30% of cases. Basal cell and basosquamous carcinoma are anti-Ber-EP4 positive in almost all cases. Malignant mesothelioma (epithelioid and
biphasic) is anti-Ber-EP4 positive in 4%-26% of the cases. The staining is usually focal, but may occasionally be widespread. Synovial sarcoma
(epithelioid and biphasic) and desmoplastic small round cell tumor stain with anti-Ber-EP4 in most cases. Seminoma, embryonal carcinoma, yolk
sac tumor, and choriocarcinoma reveal anti-Ber-EP4 positivity in a minor proportion of cases. The lack of reactivity in the majority of malignant
mesotheliomas can, in an appropriate panel, be utilized to discriminate between this tumor and adenocarcinoma.
Pleura: Adenocarcinoma vs. Mesothelioma
Ber-EP4 Calretinin CK 5&6 D2-40 HBME-1 Caldesmon CEA TAG-72 E-cadherin TTF-1
Adenocarcinoma
+
-
-
-
-
-
+
+
+
+
Mesothelioma
-
+
+
+
+
+
-
-
-
-
Carcinomas
Ber-EP4
MOC-31
Hep-Par1
CK Cocktail
CK, 34βE12
CAM 5.2
CK 19
Basal Cell Carcinoma
+
+/-
-
+
+
+/-
-
Skin Squamous Carcinoma
-
-/+
-
+
+
-
-
Merkel Cell Carcinoma
+
+
-
+
-/+
+
-
Hepatocellular Carcinoma
-
-
+
-
-
+
-
Cholangio Carcinoma
+
+
-
+
+
+
+
Sarcomatoid Carcinoma
+
-
-
+
+
+/-
-
Reference
1. Latza, et al. J Clin Pathol. 1990; 43:213-19.
2. Ma, et al. Am J Clin Pathol. 1993; 99(5):551-7.
3. Carella, et al. Am J Surg Pathol. 2001; 25(1):43-50.
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