Advanced Solutions
for Advanced Pathology
Desmin (D33)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45204 IMPATH Desmin RTU M (D33)
50 Tests
44279 Desmin RTU M (D33)
7 ml Ready To Use
44580 Desmin 0,1 M (D33)
100 µl liquid Concentrated
44581 Desmin 1 M (D33)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Skeletal Muscle
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-desmin detects a protein that is expressed by cells of normal smooth, skeletal, and cardiac muscles. The light microscope has suggested that
desmin is primarily located at or near the periphery of Z lines in striated muscle fibrils. In smooth muscle, desmin interconnects cytoplasmic dense
bodies with membrane-bound dense plaques. Anti-desmin reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and
perivascular cells of glomus tumors of the skin (if they are of myogenic nature). This antibody is used to demonstrate the myogenic components/
derivation of tumors.
Soft Tissue Tumor
Desmin CK Cocktail
EMA
MS Actin SM Actin
CD34
CD99
ALK-1
Epithelioid Sarcoma
-
+
+
-/+
-
+
-
-
PNET/ES
-
-/+
-
-
-
-
+
-
Desmoplastic Small Round
Cell
+
+
-
-
-
-
-
-
Myofibroblastic Tumor
+/-
-
-
+
+
-
-
+
Spindle Cell Tumors
Desmin β-Catenin PGP 9.5 CD117 S-100 Myogenin CD34
CK
Cocktail
Calponin BCL2
Spindle Cell Carcinoma
-
+/-
+
-
-
-
-
+
-
-
Neurofibroma
-
-
+
-
+
-
-
-
-
+
Rhabdomyosarcoma
+
-
-
+
-
+
-
-
-
+
Endometrial Stromal Tumor
-
+/-
+
-
-
-
-
-
+
-
Smooth Muscle
+
-
-
-
-
-
-
-
+
-
Fibromatosis
-
+
+
-
-
-
-
-
-
-
GIST
-
-
-
+
-
-
+
-
-
+
Schwannoma
-
-
-
-
+
-
-
-
-
+
Leiomyosarcoma
+
-
-
-
-
+/-
-
-/+
+
-
MPNST
-
-
+
+
-
-/+
-
+(focal)
Reference
1. Altmannsberger M, et al. Am J Pathol. 1985; 118:85-95.
2. Attanoos RL, et al. Histopathology. 2003 Sep; 43(3):231-8.
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