Antibodies for
Immunohistochemistry
Factor VIII-R Ag. (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45207 IMPATH Factor VIII RTU R (Poly)
50 Tests
44286 Factor VIII RTU R (Poly)
7 ml Ready To Use
44594 Factor VIII 0,1 R (Poly)
100 µl liquid Concentrated
44595 Factor VIII 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Placenta
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-factor VIII-related antigen reacts with endothelial cells. This antibody has helped to establish the endothelial nature of some lesions of
disputed histogenesis, e.g. Kaposi’s sarcoma and cardiac myxoma. This antibody is widely used for differentiating vascular lesions from those
of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation react with this antigen.
Skin: Spindle Cell Tumors
Factor VIII
FLI-1
HHV-8 D2-40 SM Actin MS Actin NGFR CD10
CK
Cocktail
S-100
Spindle Squamous Cell
Carcinoma
-
-
-
+
-
-
-
-
+
-
Spindle Cell Melanoma
-
+
-
+
-
-
+
-
-
+
Atypical Fibroxanthomas
-
-
-
-
+
+
-
+
-
-
Peripheral Nerve Sheath
-
-
-
+
-
+
-
-
-
+/-
Smooth Muscle
-
-
-
-
+
+
-
-
-
-
Angiosarcoma
+
+
-
+/-
-
-
-
-
-
-
Hemangioma
+
+
-
-
+
-
-
-
-
-
Kaposi's Sarcoma
+
+
+
+
+
-
-
-
-
-
Reference
1. Wick MR, et al. Lab Invest. 1985; 52:75A.
2. Bhawan J, et al. Cancer. 1985; 55:570-576.
3. Ansell J, et al. Cancer. 1982; 50:1506-1512.
4. Fulling KH, et al. Cancer. 1983; 51:1107-1118.
5. Bian XW, et al. Anal Quant Cytol Histol. 2000 Jun; 22(3):267-74.
6. Yamamoto T, et al. Pathol Int. 1996 May; 46(5):364-71.
7. Zatterstrom UK, et al. Head Neck. 1995 Jul-Aug; 17(4):312-8.
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