Antibodies for
Immunohistochemistry
Cytokeratin Cocktail (AE1 & AE3)
Mouse Cocktail Antibody
Cat. No. Description
Volume
45140 IMPATH Cytokeratin Cocktail RTU M (AE1 & AE3) 50 Tests
44277 Cytokeratin Cocktail RTU M (AE1&AE3)
7 ml Ready To Use
44575 Cytokeratin Cocktail 0,1 M (AE1&AE3)
100 µl liquid Concentrated
44576 Cytokeratin Cocktail 1 M (AE1&AE3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Breast, Colon, Lung, Skin
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG1/k & IgG1/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin cocktail, (AE1 & AE3) is well-suited to distinguish epithelial carcinoma from non-epithelial malignancies and is used to aid
epithelial tumor classification. This antibody has been used to characterize the source of various neoplasms and to study the distribution
of keratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains
cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Small, Round Blue Cell Tumors
CK
Cocktail
MS Actin SM Actin Myogenin CD99
CD57
FLI-1
CD45 Vimentin INI-1
Lymphoblastic Lymphoma
-
-
-
-
+
-
+
+
+
+
Rhabdomyosarcoma
-
-/+
-/+
+
-
-
-
-
+
+
Neuroblastoma
-
-
-
-
-
+
-
-
+
+
Embryonal Carcinoma
+
-
-
-
-
+
-
-
-
+
PNET/ES
-/+
-
-
-
+
+
+
-
+
+
DSRCT
+
-
-
-
-
+/-
+
-
+
+
Medulloblastoma
-
-
-
-
-
+
-
-
-
+
Soft Tissue Tumor
CK
Cocktail
S-100 MS Actin SM Actin CD34
TLE-1
A1AT
CD99
TFE-3 ALK-1
Synovial Sarcoma
+
-
-
-
-
+
-
+
-
-
Epithelioid Sarcoma
+
-
-/+
-
+
-
-
-
-
-
Clear Cell Sarcoma
-
+
-
-
-
-
-
-
-
-
PNET/ES
-/+
+
-
-
-
-
-
+
-
-
Desmoplastic Small Round
Cell
+
-
-
-
-
-
-
-
-
-
Myxoid Chondrosarcoma
-
+/-
-
-
-/+
-
-
-
-
Alveolar Soft Part Sarcoma
-
-
+
+
-
-
-
-
+
-
PEComa
-
-
-
+
-
-
-
-
-
-
Fibrous Histiocytoma
-
-
-
-
-
-
+
-
-
-
Inflammatory
Myofibroblastic Tumor
-
-
+
+
-
-
-
-
-
+
Reference
1. Battifora H. Am J Surg Pathol. 1988; 12:24.
106
