Advanced Solutions
for Advanced Pathology
Cytokeratin, HMW (AE3)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45301 IMPATH Cytokeratin HMW RTU M (AE3)
50 Tests
44275 Cytokeratin, HMW RTU M (AE3)
7 ml Ready To Use
44571 Cytokeratin, HMW 0,1 M (AE3)
100 µl liquid Concentrated
44572 Cytokeratin, HMW 1 M (AE3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Bladder, Prostate, Salivary gland
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin, high molecular weight (AE3) is capable of recognizing all basic keratins; therefore, it is a broadly reactive antibody staining most
epithelia and their neoplasms. Members of the acidic and basic subfamilies are found together in pairs. Since each epithelium contains at least
one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify
neoplasms derived from such epithelium.
Carcinomas
CK, HMW CK 7
CK 20 CK, LMW CK 5
TTF-1 GCDFP-15 pCEA Hep-Par1 RCC
Hepatocellular Carcinoma
-
-
-
-
-
+
(cytoplasmic)
-
+
+
-
Renal Cell Carcinoma
-
-
-
+
-
-
-
-
-
+
Bladder Carcinoma
+
+
+
+
-
-
-
+
-
-
Salivary Gland Carcinoma
+
+
-
+
+
-
+
-
-
Thyroid Carcinoma
+
+
-
+
-
+
-
-
-
-
Spindle Cell Carcinoma
+
-
-
-
-
-
-
-
Breast Carcinoma
+
+
-
+
-
-
+
-
-
-
Lung Adenocarcinoma
+
+
-
+
-
+
-
+
-
-
Colorectal Adenocarcinoma
-
-
+
+
-
-
-
+
-
-
Prostate Adenocarcinoma
-
-
-
+
-
-
-
-
-
-
Transitional Cell Carcinoma
+
+
+
+
+
-
-
-
-
-
Ovarian Carcinoma,
Non Mucinous
+
+
-
+
+
-
-
-
-
-
Pancreatic Carcinoma
+/-
+
-
+
-
-
-
+
-
-
Squamous Cell Carcinoma
+
-
-
+
+
-
-
-
-
-
Skin: Pagetoid Tumors
CK, HMW
CK, LMW
S-100
CEA
Vimentin
Melanoma
-
-
+
-
+
Paget’s Disease
-
+
-/+
+
-
Bowen’s Disease
+
+
-
-
-
Reference
1. Tyler CR. Arch Pathol Lab Med. 1978; 102:113.
2. Weiss RA, et al. J Cell Biol. 1984; 98:1397.
107
