Advanced Solutions
for Advanced Pathology
FLI-1 (MRQ-1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44289 FLI-1 RTU M (MRQ-1)
7 ml Ready To Use
44600 FLI-1 0,1 M (MRQ-1)
100 µl liquid Concentrated
44601 FLI-1 1 M (MRQ-1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Primitive neuroectodermal tumor
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Ewing sarcoma/peripheral primitive neuroectodermal tumor (ES/pPNET) is a rare primary tumor of the bone/soft tissue that resembles other
undifferentiated tumors. The differential diagnosis of undifferentiated tumors of the soft tissue includes blastemal Wilms’ tumor, rhabdoid tumor,
neuroblastoma, lymphoma, clear cell sarcoma, small cell carcinoma, synovial sarcoma (SS), neuroblastoma, desmoplastic small round cell tumor
(DSRCT), and ES/pPNET. The Fli-1 gene and Fli-1 protein are best known for their critical role in the pathogenesis of ES/pPNET. Fli-1 is normally
expressed in endothelial cells and in hematopoietic cells, including T-lymphocytes. The immunohistochemical detection of Fli-1 protein has been
shown in two recent studies to be valuable in the discrimination of ES/pPNET from most of its potential mimics, with the notable exception of
lymphoblastic lymphoma.
Small, Round Blue Cell Tumors
FLI-1 MS Actin SM Actin Myogenin CK
Cocktail
CD99 PGP 9.5 CD45 Vimentin CD57
Lymphoblastic Lymphoma
+
-
-
-
-
+
+
+
-
Rhabdomyosarcoma
-
-/+
-/+
+
-
-
+
-
+
-
Neuroblastoma
-
-
-
-
-
-
+
-
+
+
Embryonal Carcinoma
-
-
-
-
+
-
+
-
-
+
PNET/ES
+
-
-
-
-/+
+
+
-
+
+
DSRCT
+
-
-
-
+
-
-
-
+
+/-
Medulloblastoma
-
-
-
-
-
-
-
-
+
Skin: Spindle Cell Tumors
FLI-1 GLUT1 CD99 Factor VIII
HHV-8 CK 8 & 18 CD34
NGFR Collagen
IV
D2-40
Spindle Cell Melanoma
+
-
-
-
-
-
-
+
-
+
Angiosarcoma
+
-
-
+
-
-
+
-
+/-
+/-
Solitary Fibrous Tumor
-/+
-
+/-
-
-
-
+
-
-
-
Hemangioma
+
+
-
+
-
-
+
-
+
-
Hemangioendothelioma
+
-
-
-
-
+
+
-
-
-
Kaposi's Sarcoma
+
-
-
+
+
-
+
-
+/-
+
Reference
1. Mhawech-Fauceglia P, et al. Histopathology. 2006 Dec; 49(6):569-75.
2. Kuroda N, et al. Med Mol Morphol. 2006 Dec; 39(4):221-5. Epub 2006 Dec 21.
3. Blind C, et al. J Clin Pathol. 2008 Jan; 61(1):79-83. Epub 2007 Apr 5.
4. Ellison DA, Parham DM, Bridge J, Beckwith JB. Hum Pathol. 2007 Feb; 38(2):205-11. Epub 2006 Nov 28.
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