Advanced Solutions
for Advanced Pathology
Napsin A (MRQ-60)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45241 IMPATH Napsin A RTU M (MRQ-60)
50 Tests
44351 Napsin A RTU M (MRQ-60)
7 ml Ready To Use
44722 Napsin A 0,1 M (MRQ-60)
100 µl liquid Concentrated
44723 Napsin A 1 M (MRQ-60)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Kidney, Lung Adenocarcinoma,
Renal Cell Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. There are two closely related napsins, napsin A and
napsin B. Napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies
revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes
and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung
adenocarcinomas from adenocarcinomas of other organs.
Breast vs. Lung vs. Prostate Carcinoma
Napsin A
GCDFP-15
Mammaglobin
PSA
TTF-1
Breast Carcinoma
-
+
+
-
-
Lung Carcinoma
+
-
-
-
+
Prostate Carcinoma
-
-
-
+
-
Pleura: Adenocarcinoma vs. Mesothelioma
Napsin A Calretinin CK 5&6 HBME-1 WT1 Caldesmon Ep-CAM TTF-1 TAG-72
CEA
Adenocarcinoma
+
-
-
-
-
-
+
+
+
+
Mesothelioma
-
+
+
+
+
+
-
-
-
-
Lung Squamous Cell Carcinoma vs. Adenocarcinoma
Napsin A
TTF-1
p63
CK 5&6
SOX-2
Desmocollin3
Lung Squamous Cell
Carcinoma
-
-
+
+
+
+
Adenocarcinoma
+
+
-
-
-
-
Reference
1. Jagirdar J. Arch Pathol Lab Med. 2008; 132:384-96.
2. Dejmek A, et al. Diagn Cytopathol. 2007; 35:493-7.
3. Inamura K, et al. Am J Surg Pathol. 2005; 29:660-5.
4. Bishop JA, et al. Hum Pathol. 2010; 41:20-5.
5. Ye J, et al. Appl Immunohistochem Mol Morphol. 2011; 19:313–317.
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