Antibodies for
Immunohistochemistry
Myosin, Smooth Muscle (SMMS-1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45240 IMPATH Myosin Smooth Muscle RTU M (SMMS-1)
50 Tests
44350 Myosin, Smooth Muscle RTU M (SMMS-1)
7 ml Ready To Use
44720 Myosin, Smooth Muscle 0,1 M (SMMS-1)
100 µl liquid Concentrated
44721 Myosin, Smooth Muscle 1 M (SMMS-1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Breast
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Smooth Muscle Myosin, heavy chain (SMMS-1) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the
smooth muscle cells. SMMS-1 is also a myoepithelium-associated protein. Anti-SMMS-1 is a mouse monoclonal antibody to smooth muscle
myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells.
It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the
myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.
Breast Carcinoma in-situ vs. Infiltrating Breast Carcinoma
SM Myosin
Calponin
p63
Breast Carcinoma
in-situ
(Myoepithelial Cells)
+
+
+
Infiltrating Breast
Carcinoma
-
-
-
Spindle Cell Tumors
SM
Myosin β-Catenin PGP 9.5 DOG1
CD34
CK
Cocktail
Calponin ALK MS Actin CD56
Myofibroblastic Tumor
-
-
-
-
-
-
+
+
+
+
Spindle Cell Carcinoma
-
+/-
+
-
-
+
-
-
-
-
Neurofibroma
-
-
+
-
-
-
-
-
-
+
Rhabdomyosarcoma
-
-
-
-
-
-
-
-
+
-
Endometrial Stromal Tumor
-
+/-
+
-
-
-
+
-
+
-
Smooth Muscle
-
-
-
-
-
-
+
-
+
-
Fibromatosis
-
+
+
-
-
-
-
-
-
-
GIST
-
-
-
+
+
-
-
-
-
-
Schwannoma
-
-
-
-
-
-
-
-
-
+
Leiomyosarcoma
+
-
-
-
-
-/+
+
-
+
+
Reference
1. Werling RW, et al. Am J Surg Pathol. 2003 Jan; 27(1):82-90.
2. Agoff SN, et al. Appl Immunohistochem Mol Morphol. 2001 Jun; 9(2):164-9.
3. Popnikolov NK, et al. Am J Clin Pathol. 2003 Aug; 120(2):161-7.
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