Antibodies for
Immunohistochemistry
Nerve Growth Factor Receptor (NGFR) (MRQ-21)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45244 IMPATH NGFR RTU M (MRQ-21)
50 Tests
44354 NGFR RTU M (MRQ-21)
7 ml Ready To Use
44728 NGFR 0,1 M (MRQ-21)
100 µl liquid Concentrated
44729 NGFR 1 M (MRQ-21)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Breast
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
NGFR is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or tumor derivatives such as melanocytes,
melanomas, neuroblastomas, pheochromocytomas, neurofibromas, and neurotized nevi (type C melanocytes). Anti-NGFR has been shown to
be a reliable marker for desmoplastic and neurotropic melanoma. All reported cases of desmoplastic melanomas are positive with this antibody.
This staining property of desmoplastic melanoma cells can be useful in diagnosing challenging cases. It is now apparent that expression of NGFR
is ubiquitous and not limited to the nervous system, being expressed in mature nonneural cells such as perivascular cells, dental pulp cells,
lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR
labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and thus is an aid in the diagnosis of malignancy in the
breast.
Skin: Spindle Cell Tumors
NGFR
FLI-1
Collagen IV S-100
CD10
Factor XIIIa
CD34
D2-40
Spindle Cell Melanoma
+
+
-
+
-
-
-
+
Dermatofibrosarcoma
Protuberans
+
-
-
-
+/-
-
+
-
Dermatofibroma Fibrous
Histiocytoma
-
-
-
-
+
+
-
-
PNST
+
+
+
CNS Tumors
NGFR GFAP Neuro-
filament
Synapto-
physin S-100
CK
Cocktail
PR
EMA Vimentin INI-1
Astrocytoma
+
+
-
-
+
-
-
-
+
+
Ependymoma
+
+
-
-
+
-
-
-
-/+
+
Choroid Plexus Carcinoma
-
-/+
-
+
+
+
-
-
+
Central Neurocytoma
+
-
-
+
-
-
-
-
-
+
Neuroblastoma
+
+/-
+
+
+/-
-
-
-
+
+
Pineocytoma
-
-
-
+
-
-
-
-
+
Meningioma
-
-
-
-
-
-
+
+
+
+
Schwannoma
+
+
-
-
+
-
-
-
+
+
Metastatic Carcinoma
-
-
-
-
-
+
-/+
+
-/+
+
Reference
1. Laskin WB, et al. Hum Pathol. 2000 Oct; 31(10):1230-41.
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