Antibodies for
Immunohistochemistry
C4d (SP91)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45296 IMPATH C4d RTU R (SP91)
50 Tests
44210 C4d RTU R (SP91)
7 ml Ready To Use
44445 C4d 0,1 R (SP91)
100 µl liquid Concentrated
44446 C4d 1 R (SP91)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Acute rejected kidney transplant,
Lymph node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
C4d is a stable split product remnant of classical complement activation which becomes covalently bound to endothelium and basement
membrane after induction of the classical antibody-induced pathway. As an established marker of antibody-mediated acute renal allograft
rejection (AR) and its proclivity for endothelium, this component can be detected in peritubular capillaries in both chronic renal allograft rejection
as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant
predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of AR that may serve to
warrant prompt and aggressive anti-rejection treatment.
Reference
1. Jianghua C, et al. Clin Transplant. 2005; 19:785-91.
2. Kayler LK, et al. Transplantation. 2008; 85:813-20.
3. Ranjan P, et al. Nephrol Dial Transplant. 2008; 23:1735-41. Epub 2007 Dec 8.
4. Nadasdy GM, et al. Hum Pathol. 2005; 36:1178-85.
5. Seemayer CA, et al. Nephrol Dial Transplant. 2007; 22:568-76.
6. Bouron-Dal Soglio D, et al. Hum Pathol. 2008; 39:1103-10.
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