Advanced Solutions
for Advanced Pathology
Caldesmon (E89
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45272 IMPATH Caldesmon RTU R (E89)
50 Tests
44214 Caldesmon RTU R (E89)
7 ml Ready To Use
44452 Caldesmon 0,1 R (E89)
100 µl liquid Concentrated
44453 Caldesmon 1 R (E89)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Appendix, Breast
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Caldesmon is a regulatory protein found in smooth muscle and other tissues which interacts with actin, myosin, tropomyosin, and calmodulin.
Anti-caldesmon labels smooth muscle and tumors of smooth muscle, myofibroblastic, and myoepithelial differentiation. Anti-caldesmon has also
been used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary.
Small, Round Blue Cell Tumors
Caldesmon MS Actin SM Actin Myogenin Myoglobin Calponin PGP 9.5 CD57 Vimentin INI-1
Leiomyosarcoma
+
+
+
-
-
+
-
+/-
+
Rhabdomyosarcoma
-
+
-
+
+
-
+
-
+
+
PEComa
Caldesmon HMB-45 MART-1 CD63
S-100 Tyrosinase SM Actin Calponin Desmin CD68
Angiomyolipoma
+
+
+
+
-
-
+
+
-
+
Lymphangiomyomatosis
+
+
+
+
-
-
+
+
-
-
Extrapulmonary Clear Cell
Tumor
-
+
+
+
+
-
+
-
-
-
Primary Cutaneous
PEComa
-
+
+
+
-
-
-
-
-
+/-
Pulmonary Clear Cell Sugar
Tumor
-
+
+
+
+/-
-
-
-
-
+/-
Spindle Cell Tumors
Caldesmon SM Actin MS Actin Desmin
Calponin Myogenin CK
Cocktail
EMA ALK-1 S-100
Myofibroblastic Tumor
+
+
+
+
+
-
-
-
+
-
Spindle Cell Carcinoma
-
-
-
-
-
-
+
+/-
-
-
Neurofibroma
-
-
-
-
-
-
-
-
-
+
Rhabdomyosarcoma
-
-
+
+
-
+
-
+/-
-
-
Endometrial Stromal Tumor
-
+
+
-
+
-
-
-
-
-
Smooth Muscle
+
+
+
+
-
-
-
-
-
-
Reference
1. Comin CE, Saieva C, Messerini L. Am J Surg Pathol. 2007 Aug; 3198):1139-48.
2. Watanabe K, Kusakabe T, Hoshi N, Saito A, Suzuki T. Hum Pathol. 1999 Apr; 30(4):392-6.
3. McCluggage WC. Adv Anat Pathol. 2004 May; 11(3):162-71.
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