Antibodies for
Immunohistochemistry
Calponin-1 (EP798Y
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45171 IMPATH Calponin-1 RTU R (EP798Y)
50 Tests
44215 Calponin RTU R (EP798Y)
7 ml Ready To Use
44454 Calponin 0,1 R (EP798Y)
100 µl liquid Concentrated
44455 Calponin 1 R (EP798Y)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Appendix
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Calponin is a 34 kD polypeptide that interacts with actin, tropomyosin, and calmodulin. It is involved in smooth muscle contraction mechanism
and is restricted exclusively to smooth muscle tissue. Anti-calponin has been found to be useful in staining myoepithelium and is therefore useful
for differentiating benign sclerosing adenosis of the breast from infiltrating ductal carcinoma. Calponin positivity has also been noted in malignant
myoepithelioma and pleomorphic adenoma of salivary gland origin as well as angiomatoid malignant fibrous histiocytoma.
Myoepithelial Lesions: Malignant vs. Benign
Calponin
CK
Cocktail
MS Actin SM
Myosin S-100
GFAP EMA CK 14
p63
Desmin
Malignant Myoepithelioma
+
+
+
+
+
+/-
+
+
-
-
Benign Myoepithelium
+
+
+
+
+
+
+
+
+
-
Soft Tissue Tumors
Calponin MS Actin SM Actin Myogenin CK
Cocktail
CD99
FLI-1 PGP 9.5 CD57
INI-1
Leiomyosarcoma
+
+
+
-
-/+
-
-
-
+/-
Rhabdomyosarcoma
-
-/+
-/+
+
-
-
-
+
-
+
PNET/ES
-
-
-
-
-/+
+
+
+
+
+
Breast Carcinoma In-Situ vs. Infiltrating Breast Carcinoma
Calponin
SM Myosin
p63
Sclerosing adenosis
+
+
+
Infiltrating Breast
Carcinoma
-
-
-
Spindle Cell Tumors
Calponin β-Catenin PGP 9.5 ALK
CK
Cocktail
CD56 Myogenin SM
Myosin Caldesmon Desmin
Myofibroblastic Tumor
+
-
-
+
-
+
-
-
+
+
Endometrial Stromal Tumor
+
+/-
+
-
-
-
-
-
-
-
Smooth Muscle
+
-
-
-
-
-
-
-
+
+
Leiomyosarcoma
+
-
-
-
-/+
+
+/-
+
+
+
Reference
1. Wang NP, et al. Appl. Immunohistochem. 1997; 5(3):141-151.
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