Antibodies for
Immunohistochemistry
Calcitonin (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45170 IMPATH Calcitonin RTU R (Poly)
50 Tests
44213 Calcitonin RTU R (Poly)
7 ml Ready To Use
44450 Calcitonin 0,1 R (Poly)
100 µl liquid Concentrated
44451 Calcitonin 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Thyroid Medullary Carcinoma
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Immunohistochemical staining with anti-calcitonin antibody has proven to be an effective way of demonstrating calcitoninproducing cells in the
thyroid. C-cell hyperplasia and medullary thyroid carcinomas stain positive for calcitonin. Studies of calcitonin have resulted in the identification
of a wide spectrum of C-cell proliferative abnormalities.
Thyroid: Malignant vs. Benign
Calcitonin
Thyroglobulin
CK 19
Galectin-3
TTF-1
HBME-1
Papillary Carcinoma
-
+
+
+
+
+
Follicular Carcinoma
-
+
-/+
+
+
+/-
Medullary Carcinoma
+
-
+/-
-
+
+
Benign Thyroid
-
+
-
-
+
-
Differential Diagnosis of Parathyroid vs. Thyroid Tumors
Calcitonin
Chromogranin A Synaptophysin
PTH
S-100
TTF-1
Parathyroid Tumors
-
+
+
+
-
-
Follicular Thyroid Tumors
-
-
-
-
+/-
+
Medullary Thyroid
Cacinoma
+
+
+
-
-
+
Reference
1. Copp DH, et al. Endocrinology. 1962; 70:638-649.
2. Kameda Y, et al. Cell Tissue Res. 1980; 206:403-415.
3. Coombes RC, et al. Lancet. 1974; 1:1080-1083.
4. Dayal Y, et al. Cancer. 1979; 43:1331-13385.
5. DeLellis, RA et al. Am J Clin Pathol. 1978; 7(4):587-29.
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