Advanced Solutions
for Advanced Pathology
Calretinin (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45172 IMPATH Calretinin RTU R (Poly)
50 Tests
44216 Calretinin RTU R (Poly)
7 ml Ready To Use
44456 Calretinin 0,1 R (Poly)
100 µl liquid Concentrated
44457 Calretinin 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Nuclear
Control
Mesothelioma
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-calretinin has been shown to be useful in differentiating mesothelioma from adenocarcinomas of the lung and other sources. Anti-calretinin
has also demonstrated utility in differentiating adrenal cortical neoplasms from pheochromocytomas.
Adrenocortical Tumors
Calretinin
Inhibin
MART-1
Synaptophysin Chromogranin A
CD56
Pheochromocytoma
-
-
-
+
+
+
Carcinoma
-
-
-
-
-
-
Adenoma
+
+
+
-/+
-
+
Lung Adenocarcinoma vs. Mesothelioma
Calretinin CK 5&6 D2-40 HBME-1 Caldesmon TAG-72
CEA Ep-CAM E-cadherin TTF-1
Adenocarcinoma
-
-
-
-
-
+
+
+
+
+
Mesothelioma
+
+
+
+
+
-
-
-
-
-
Soft Tissue Tumor
Calretinin CK Cocktail
EMA SM Actin CD34
TLE-1
Desmin
S-100
CD56
Synovial Sarcoma
-
+
+
-
-
+
-
-
+
Myxoid Chondrosarcoma
-
-
-
-
-/+
-
-
+/-
-
PEComa
-
-
-
+
-
-
+/-
-
-
Sex Cord Stromal Tumors
Calretinin
Inhibin
CD99
CK 7
EMA
Vimentin
MART-1
Granulosa Cell Tumors
+
+
+
-
-
+
+
Sertoli-Leydig Cell Tumors
+
+
+
+
-
+
+
Gonadoblastomas
+
+
+
-
-
+
-
Reference
1. Barberis MC, et al. Acta Cytol. 1997 Nov-Dec; 41(6):1757-61.
2. Doglioni C, et al. Am J Surg Pathol. 1996 Sep; 20(9):1037-46.
3. Leers MP, et al. Histopathology. 1998 Mar; 32(3); 209-16.
4. Ordonez NG. AM J Surg Pathol. 1998 Oct; 22(10):1203-14.
5. Ordonez NG. Mod Pathol. 1998 Oct; 11(10):929-33.
6. Abutaily AS, et al. J Clin Pathol. 2002 Sep; 55(9):662-8
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