Antibodies for
Immunohistochemistry
CD1a (EP3622
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45176 IMPATH CD1A RTU R (EP3622)
50 Tests
44225 CD1a RTU R (EP3622)
7 ml Ready To Use
44472 CD1a 0,1 R (EP3622)
100 µl liquid Concentrated
44473 CD1a 1 R (EP3622)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Skin, Thymus
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD1a is a non-polymorphic major histocompatibitity complex class I-related cell surface glycoprotein (45 to 55 kDa) and is expressed in
association with β-microglobulin. In normal tissues, anti-CD1a reacts with cortical thymocytes, Langerhans cells, interdigitating dendritic cells,
and rare antigen-presenting cells of the lymph node. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic
sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with
antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently
expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in
pulmonary neoplasms. Anti-CD1a was reported to be a new marker for perivascular epithelial cell tumor (PEComa).
Lymph Node
CD1a
CD68
S-100
CD163
CD21/CD35
CD14
Reactive Histiocytosis
-
+
-
-
-
+
Langerhans Cell
Histiocytosis
+
+
+
+
-
+
Sinus Histiocytosis with
Massive Lymphadenopathy
-
+
+
+
-
+
Follicular Dendritic Cell
Sarcoma
+/-
-
-
-
+
-
Dermatopathic
Lymphadenitis
+
-
+
+
-
-
Histiocytic Proliferation
CD1a
S-100
CD68
Vimentin
CD163
Factor XIIIa
HAM-56
Juvenile Xanthogranuloma
-
-
+
+
+
+
+
Langerhans Cell
Histiocytosis
+
+
+
+
+
-
+
Dermatofibroma
-
-
+
+
-
+
-
Reference
1. Pinkus GS, et al. Am J Clin Pathol. 2002 Sep; 118(3):335-43.
2. Laguens G, et al. Immunol Lett. 2002 Dec 3; 84(3):159-62.
3. Pileri SA, et al. Histopathology. 2002 Jul; 41(1); 1-29.
4. Schmuth M, et al. Am J Clin Pathol. 2001 Jul; 1169(1):72-8.
5. Shiyong L, et al. Arch Pathol Lab Med. 2001 Jul; 125(7):958-60.
6. Goteri G, et al. J Clin Pathol. 2003 Jun; 56(6):453-8.
7. Deguchi M, et al. Arch Dermatol Res. 2002 Oct; 294(7):297-302. Epub 2002 Jul 27.
44
