Advanced Solutions
for Advanced Pathology
CD2 (MRQ-11)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45177 IMPATH CD2 RTU M (MRQ-11)
50 Tests
44226 CD2 RTU M (MRQ-11)
7 ml Ready To Use
44474 CD2 0,1 M (MRQ-11)
100 µl liquid Concentrated
44475 CD2 1 M (MRQ-11)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD2 is one of the earliest T-cell lineage restricted antigens to appear during T-cell differentiation and only rare CD2+ cells can be found in the
bone marrow. Anti-CD2 is a pan-T-cell antigen marker. Anti-CD2 is therefore useful for the identification of virtually all normal T-lymphocytes. It
is also very useful in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and mature T-cell lymphomas and
leukemias. As with other pan-T-cell antigens, CD2 may be aberrantly deleted in some neoplastic T-cell populations, especially peripheral T-cell
lymphomas. When combined with anti-CD25, anti-CD2 may assist in the diagnosis of systemic mastocytosis and mastocytic leukemia.
T-cell Lymphomas
CD2
CD45
CD3
CD4
CD5
CD7
CD8
CD25 CD45RO PD-1
Angioimmunoblastic
+
+
+
+
+
+
-
+
+
+
Lymphoblastic
+/-
+
+
+/-
+
+
+/-
+
+
-
Subcutaneous
Panniculitis-Like
+
+
+
-
+
+
+/-
-
+
-
NK
+
+
+
-
-
-/+
-
+
+
-
Cutaneous
+
+
+
+
-
+
-
-
-
-/+
Peripheral, NOS
+
+
+
+/-
+/-
+/-
-/+
+
+
-
Mycosis Fungoides
+
+
+
+
+
-
-
+
+
-
Mastocytosis
CD2
Tryptase
CD117
CD25
CD163
Mastocytosis
+
+
+
+
-
Mastocytic Leukemia
+
+
+
+
-
Reactive Mast Cells
-
+
+
-
+
Reference
1. Aguilera NS, et al. Arch Pathol Lab Med. 2006 Dec; 130(12):1772-9.
2. Barrionuevo C, et al. Appl Immunohistochem Mol Morphol. 2007 Mar; 15(1):38-44.
3. Bovenschen HJ, et al. Br J Dermatol. 2005 Jul; 153(1):72-8.
4. Foon KA, Todd RF. Blood. 1986; 68:1-31.
5. Gonzalez L, et al. Journal of Comparative Pathology. 2001; 125:41-7.
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