Advanced Solutions
for Advanced Pathology
CD4 (EP204
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44238 CD4 RTU R (EP204)
7 ml Ready To Use
44498 CD4 0,1 R (EP204)
100 µl liquid Concentrated
44499 CD4 1 R (EP204)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Lymph Node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
CD4 is a 55 kD glycoprotein expressed on the surface of T-helper/regulatory T-cells, monocytes, macrophages, and dendritic cells. Anti-CD4
is used in the immunophenotyping of lymphoproliferative disorders. The majority of peripheral T-cell lymphomas are derived from the T-helper/
regulatory cell subset so that most mature T-cell neoplasms are CD4+ CD8-. As with other T-cell antigens, CD4 may be aberrantly expressed
in neoplastic T-cells so that the evaluation of such tumors requires the application of a panel of markers in order to identify tumors with CD4
aberrant expression.
T-cell Lymphomas
CD4
CD45
CD2
CD3
CD5
CD7
CD8
CD25 CD45RO PD-1
Angioimmunoblastic
+
+
+
+
+
+
-
+
+
+
Lymphoblastic
+/-
+
+/-
+
+
+
+/-
+
+
-
Subcutaneous
Panniculitis-Like
-
+
+
+
+
+
+/-
-
+
-
NK
-
+
+
+
-
-/+
-
+
+
-
Cutaneous
+
+
+
+
-
+
-
-
-
-/+
Peripheral, NOS
+/-
+
+
+
+/-
+/-
-/+
+
+
-
Mycosis Fungoides
+
+
+
+
+
-
-
+
+
-
Histiocytic Neoplasms
CD4
CD45
CD68
Lysozyme
CD163 Factor XIIIa
CD20
CD3
Histiocytic Neoplasms
+
+
+
+
+
+
-
-
Reference
1. Leong AS-Y, Cooper K, Leong FJW-M. Manual of diagnostic antibodies for immunohistochemistry, 2nd edition 2003; Greenwich Medical Media Ltd.
2. Akiyama T, et al. Pathol Int. 2008 Oct; 58(10):626-34.
3. Lehe C, et al. Cancer Res. 2008 Aug 1; 68(15):6350-9.
4. Garcia-Herrera A, et al. J Clin Oncol. 2008 Jul 10; 26(20):3364-71. Epub 2008 Jun 9.
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