Advanced Solutions
for Advanced Pathology
CD10 (56C6)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45125 IMPATH CD10 RTU M (56C6)
50 Tests
44217 CD10 RTU M (56C6)
7 ml Ready To Use
44458 CD10 0,1 M (56C6)
100 µl liquid Concentrated
44459 CD10 1 M (56C6)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Kidney, Lymph node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) or AP
2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The antibody against CD10, common acute lymphoblastic leukemia antigen (CALLA), is a useful marker for the characterization of leukemias and
lymphomas. This antibody labels lymphoblastic, Burkitt, and follicular lymphomas. Also, anti-CD10 detects the antigen of glomerular epithelial
cells and the brush border of the proximal tubules; this characteristic may be helpful in interpreting renal ontogenesis in conjunction with other
markers. Other non-lymphoid cells that are reactive with anti-CD10 are breast myoepithelial cells, bile canaliculi, neutrophils, stromal cells of
bone marrow, and fibroblasts. Therefore, anti-CD10 has been used in classification of carcinomas, lymphomas, and leukemias.
Carcinomas
CD10
CK 7
CK 20 β-Catenin CK 5
p63
pCEA CDX-2
Villin Hep-Par1
Hepatocellular Carcinoma
+
-
-
-
-
-
+
-
-
+
Colorectal Adenocarcinoma
+
-
+
+
-
-
+
+
+
-
Transitional Cell Carcinoma
+
+
+
-
+
+
-
-
-
-
B-cell Lymphomas
CD10
CD20
CD5
BCL2
BCL6
TCL1
CD23 Cyclin D1 MUM1
Follicular
+
+
-
+
+
+
-
-
-
CLL/SLL
-
+
+
+
-
+
+
-
+
Mantle Cell
-
+
+
+
-
+
-
+
-/+
Marginal Zone
-
+
-
+
-
-
-
-
+
Burkitt
+
+
-
-
+
+
-
-
-
Diffuse Large Cell
+/-
+
-/+
+
+/-
+
-
-
+
Kidney: Renal Epithelial Tumors
CD10
RCC
PAX-2
Vimentin Ksp-cadherin Parvalbumin CD117
Ep-CAM
Clear Cell RCC
+
+
+
+
-
-
-
-
Chromophobe RCC
-/+
-/+
+
-
+
+
+
+
Oncocytoma
+/-
-
+
-
+/-
+
+
-
Reference
1. Haralambidou S, at al. J Clin Pathol. 1987; 40:490-493.
2. Mechterscheimer, et al. Am J of Pathol 1989; 134(5):961-965.
3. Hoefnagel JJ, et al. Br J Dermatol. 2003 Dec; 149(6):1183-91.
4. Sasaoka A, et al. Clin Nephrol. 2003 Nov; 60(5):305-14.
5. Hans CP, et al. Blood. 2004 Jan 1; 103(1):275-82.
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