Antibodies for
Immunohistochemistry
CD5 (EP77
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44244 CD5 RTU R (EP77)
7 ml Ready To Use
44509 CD5 0,1 R (EP77)
100 µl liquid Concentrated
44510 CD5 1 R (EP77)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Lymph Node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma
(CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis
of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes
anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in
distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
Thymus
CD5
CK 5
p63
BG8
BCL2
CD117
CD57
Thymic Carcinoma
+
+
+
+
+
+
-
Type B
3
Thymoma
-
+
+
-
-
-/+
+
B-cell Lymphomas
CD5
CD45
CD20
CD79a
BCL2
ZAP-70
CD23 Cyclin D1 MUM1
CLL/SLL
+
+
+
+
+
+
+
-
+
Mantle Cell
+
+
+
+
+
-
-
+
-
Lymphoblastic Lymphomas, B-cell vs. T-cell
CD5
TdT
CD10
PAX-5
CD20
CD19
CD3
CD7
CD117 CD1a
B-cell
-
+
+
+
+/-
+
-
-
-
+
T-cell
+/-
+
+/-
-
-
-
+
+/-
-/+
+/-
T-cell Lymphomas
CD5
CD45
CD2
CD3
CD4
CD7
CD8
CD25 CD45RO PD-1
Angioimmunoblastic
+
+
+
+
+
+
-
+
+
+
Lymphoblastic
+
+
+/-
+
+/-
+
+/-
+
+
-
Subcutaneous Panniculitis-
Like
+
+
+
+
-
+
+/-
-
+
-
NK
-
+
+
+
-
-/+
-
+
+
-
Cutaneous
-
+
+
+
+
+
-
-
-
-/+
Peripheral, NOS
+/-
+
+
+
+/-
+/-
-/+
+
+
-
Mycosis Fungoides
+
+
+
+
+
-
-
+
+
-
Reference
1. Chan JKC, et al. Histopathology. 1994; 25:517-536.
2. Kasaian, MT, et al. Proc of the Soc for Exp Bio and Med 1991; 197:226-241
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