Antibodies for
Immunohistochemistry
CD11c (5D11)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45126 IMPATH CD11c RTU M (5D11)
50 Tests
44219 CD11c RTU M (5D11)
7 ml Ready To Use
44843 CD11c 0,1 M (5D11)
100 µl liquid Concentrated
44462 CD11c 1 M (5D11)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Bone Marrow, Granulocytes,
Monocytes, Hairy Cell Leukemia
Stability
Up to 36 mo. at 2-8°C
Isotype
lgG2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Hairy cell leukemia (HCL) is a distinctive yet uncommon chronic B-cell lymphoproliferative disorder predominantly involving the bone marrow
and spleen. Bone marrow aspiration and trephine biopsy are necessary for making a definitive diagnosis and treatment of hairy cell leukemia
(HCL). However, aspiration is successful in only 10% of patients due to diffusely increased reticulin fibers in the background marrow stroma in
association with HCL infiltrates. In such situations, examination of bone marrow trephine histology together with immunohistochemical analysis
is the only available method to make a definitive diagnosis of HCL.
Korinna et al. investigated 31 bone marrow trephines with low-level HCL infiltrates and showed that the anti-CD11c (clone 5D11) was able to
detect HCL to a level of 2% tumor cells in BM biopsies. This indicates that immunohistochemical staining of formalin-fixed, decalcified bone
marrow trephine biopsies with anti-CD11c can be used both for early diagnosis of HCL and for detection of residual disease following therapy. It
is important to note that the anti-CD11c-positive interstitial macrophages, which were generally more weakly stained than the hairy cells, did not
interfere with the identification of the more strongly stained tumor cells. Among malignant lymphomas, CD11c is consistently expressed in HCL,
although it is also rarely detected in B-CLL/small lymphocytic lymphoma and splenic MZL.
Mature B-cell Neoplasms
CD11c CD25 CD103 CD123 CD10
T-bet
DBA44 TRAcP Annexin
A1 Cyclin D1
Hairy Cell Leukemia
+
+
+
+
+20% +
+/-
+/-
+
+(weak)/-
Hairy Cell Leukemia Variant
+
-
+/-
-
-
-
+/-
+/-
-
-
Splenic Marginal Zone
Lymphoma
-/+
-
-
-
-
-
+/-
+/-
-
-
Reference
1. Korinna J, et al. Pathobiology. 2008; 75:252-256.
2. Jones G, et al. Br J Hemaetol. 2011; 156:186-195.
3. Went PT, et al. Am J Surg Pathol. 2005; 29:474-478.
4. Miranda RN, et al. Modem Pathology. 2000; 13:1308-1314.
5. Marotta G, et al. Leuk Lymphoma. 2000; 37:145-149.
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