Antibodies for
Immunohistochemistry
CD14 (EPR3653
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45274 IMPATH CD14 RTU R (EPR3653)
50 Tests
44221 CD14 RTU R (EPR3653)
7 ml Ready To Use
44465 CD14 0,1 R (EPR3653)
100 µl liquid Concentrated
44466 CD14 1 R (EPR3653)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Acute Myeloid Leukemia, Appendix,
Colon, Tonsil, Lymph node
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD14 labels a 55 kDa, glycosyl-phosphatidylinositol-linked membrane protein, involved in endotoxin binding and recognition of apoptotic
cells. CD14 is expressed by monocytes and dermal dendritic cells; anti-CD14 is considered to be a macrophage-derived monocyte marker. CD14
is also present in granulocytes, endothelial, epithelial cells, and placental trophoblasts. In the spleen, CD14 can be expressed in the red pulp
and marginal zone cells, and histiocytes around sheathed capillaries. In the lymph node, true sinusoidal histiocytes, and follicular dendritic cells
stain with anti-CD14. However, other monocyte-derived cells in the lymph node, such as in sinusoidal histiocytosis with erythrophagocytosis,
macrophages associated with anthracosis, germinal center tingible body macrophages in reactive germinal centers, and diffuse large B-cell
lymphoma do not express CD14 antigen. CD14 is not expressed in plasmacytoid dendritic cells. Anti-CD14 positive histiocytes are reported as
markedly increased in DLBCL, but not in CLL/SLL, MCL, or FL.
Anti-CD14 is useful in confirming a diagnosis of massive lymphadenopathy with sinus histiocytosis (Rosai-Dorfman disease) when used in
a panel including anti-S100 and anti-CD68. Anti-CD14 can also be used for decalcified bone marrow biopsy specimens to show increased
myelomonocytic and monocytic neoplastic cells in chronic myelomonocytic leukemia and monocytic leukemia, and is very helpful in the
distinction of myeloproliferative neoplasms, myelodysplastic syndrome, and acute monocytic leukemia. This antibody is more sensitive for
leukemic monocytic cells than antibodies directed against CD163 and CD68/PG-M1.
Lymph Node
CD14
CD169
CD68
CD1a
Sinusoidal Histiocytes
+
-
-
-
Tingible Body
Macrophages
-
-
+
-
Plasmacytoid Monocytes
-
-
-
-
Langerhans Cell
Histiocytosis
+
+/-
+
+
Interdigitating DC
+/-
-
-
+
Reference
1. Gregory CD, et al. Apoptosis. 1999; 4:11-20.
2. Larregina AT, et al. Nature Immunol. 2001; 2:1151-8.
3. Ziegler-Heitbrock HW, et al. Immunol Today. 1993; 13:121-5.
4. Steiniger B, et al. Immunology. 1997; 92:307-16.
5. Buckly PJ, et al. AM J Pathol. 1987; 128:505-20.
6. Hartnell A, et al. Blood. 2001; 97:288-96.
7. Marmey B, et al. Hum Pathol. 2006; 37:68-77.
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