Advanced Solutions
for Advanced Pathology
CD15 (MMA)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45174 IMPATH CD15 RTU M (MMA)
50 Tests
44222 CD15 RTU M (MMA)
7 ml Ready To Use
44467 CD15 0,1 M (MMA)
100 µl liquid Concentrated
44468 CD15 1 M (MMA)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Hodgkin Lymphoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgM
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
A positive reaction for anti-CD15 with a membranous and perinuclear golgi zone accentuation staining pattern provides immunohistochemical
support for Reed-Sternberg cells in Hodgkin lymphoma. Also, this antibody is useful in distinguishing epithelioid mesothelioma from
adenocarcinoma when combined with other antibodies.
Hodgkin vs. Non-Hodgkin Lymphomas
CD15 CD79a CD30 Fascin Granzyme
B
BCL6
PU.1
MUM1 ALK-1
EMA
Hodgkin Lymphoma,
Classic
+
-
+
+
-
-
-
+
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
-
+
-
-
-
+
+
-/+
-
+
T-cell Rich B-cell
Lymphoma
-
+
-
-
-
+
-
+
-
-
Anaplastic Large Cell
Lymphoma
-
-
+
-
+
+/-
-
-
+
+
Skin: Adnexal Tumors
CD15
CK 7
CK 20
S-100
EMA
GCDFP-15
Merkel Cell Carcinoma
-
-
+
-
+
-
Sebaceous Tumor
+
+
-
-
-
-
Apocrine Tumor
+/-
+
-
-
+/-
+
Eccrine Tumor
-
+
-
+
+
-
Reference
1. Skubitz K, et al. Oxford Univ Press. 1989; 800-805.
2. Hsu SM, et al. Am J Clin Path. 1984; 82.
3. Pinkus GS, et al. Am J Path. 1985; 119:244-252.
4. Wieczorek R, et al. Am J Path. 1985; 121:374-380.
5. Swerdlow SH, et al. Am J Path. 1986; 85:283-282.
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