Antibodies for
Immunohistochemistry
CD16 (SP175)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45647 IMPATH CD16 RTU R (SP175)
50 Tests
45634 CD16 RTU R (SP175)
7 ml Ready To Use
45612 CD16 0,1 R (SP175)
100 µl liquid Concentrated
45613 CD16 1 R (SP175)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD16 is a biomarker associated with monocytes and natural killer (NK) cells of the lymphoid lineage. Anti-CD16 immunohistochemistry is useful
in differentially diagnosing hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from other
peripheral T-cell lymphomas, such as mucosal and cutaneous gamma delta T-cell lymphoma. It is reported that 58% of hepatosplenic gamma
delta T-cell lymphomas express CD16, and 86% of gamma delta T-cell large granular lymphocyte leukemias are immunoreactive with anti-CD16.
Mucosal and cutaneous gamma delta T-cell lymphomas usually do not express CD16 antigen. A significant decrease can be seen in the number
of granulocytes expressing CD16 in chronic myelomonocytic leukemia compared to chronic myelogenous leukemia and control bone marrow
biopsy, probably related to dysgranulopoiesis. Bone marrow biopsy immunohistochemistry can be helpful in CMML by identifying both the
monocyte expansion and the dysgranulopoiesis with anti-CD16.
NK Cell Leukemia/Lymphoma
CD16
CD2
CD3
CD56
CD57
Granzyme B Perforin
TIA-1
Aggressive NK-Cell
Leukemia
+
+
+
+
-
+
+
+
Chronic Lymphoproliferative
Disorders of NK-Cells
+
-/+
+
+/-
-/+
+
+
+
T-Cell Large Granular
Lymphocytic Leukemia
+
+
+
-
+
+
+
+
Extranodal NK/T-Cell
Lymphoma, Nasal Type
-
+
+
+
-
+
+
+
Reference
1. Liang X, Graham DK. Cancer. 2008; 112:1425-36.
2. Gibson SE, et al. Hum Pathol. 2011; 42:679-687.
3. Cooke CB, et al. Blood. 1996; 88:4265-4274.
4. Arnulf B, et al. Blood. 1998; 92:1723-1731.
5. Qubaja M, et al. Virchows Arch. 2009; 454:411-419.
56
