Advanced Solutions
for Advanced Pathology
CD19 (MRQ-36)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45175 IMPATH CD19 RTU M (MRQ-36)
50 Tests
44224 CD19 RTU M (MRQ-36)
7 ml Ready To Use
44470 CD19 0,1 M (MRQ-36)
100 µl liquid Concentrated
44471 CD19 1 M (MRQ-36)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) for AP 2-step
Polymer (Universal) 12 min
*Please refer to product insert for complete protocol.
Product Description
CD19 is present in both benign and malignant B-cells and has long been considered to be the most reliable surface marker of this lineage over a
wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells
of the interfollicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22.
However, in contrast to CD20, CD19 is also expressed in immature B-cells. Recently, Masir et al. have described the expression of CD19 in
normal lymphoid tissue and its loss in B-cell neoplasms.
Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell
neoplasms are nearly always negative, as are T-cell neoplasms.
Lymphoblastic Lymphomas, B-cell vs. T-cell
CD19
TdT
CD10
PAX-5
CD20
CD3
CD5
CD7
CD117 CD1a
B-cell
+
+
+
+
+/-
-
-
-
-
+
T-cell
-
+
+/-
-
-
+
+/-
+/-
-/+
+/-
Plasma Cells
CD19
CD138
CD79a
EMA
MUM1
CD56 Cyclin D1 CD43
CD20
Plasma Cell Neoplasm
-
+
+
+
+
+
-/+
-
-/+
Reference
1. Steinmetz OM, et al. Transplantation. 2007 Oct 15; 84(7):842-50.
2. Teng YK, et al. Arthritis Rheum. 2007 Dec; 56(12):3909-18.
3. Kimura M, et al. Int J Hematol. 2007 Jan; 85(1):41-8.
4. Masir N, et al. Histopathology. 2006 Feb; 48(3):239-46.
5. Greenberg SA, et al. Neurology. 2005 Dec 13; 65(11):1782-7.
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