Advanced Solutions
for Advanced Pathology
CD45R (MB1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44242 CD45R RTU M (MB1)
7 ml Ready To Use
44505 CD45R 0,1 M (MB1)
100 µl liquid Concentrated
44506 CD45R 1 M (MB1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Lymph node, tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
CD45R, also named MB1, is the isoform of CD45, the protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP)
family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle,
and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic
catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells and has been shown to be an
essential regulator of T- and B-cell antigen receptor signaling. CD45 functions as a phospho-tyrosine phosphatase, a vital component for efficient
tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved
intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several
members of these two families, including Lck, Fyn and Zap70, have been implicated as physiological substrates of CD45.
This antibody exhibits strong and specific reactivity with most B-lymphocytes such as follicle center cells, mantle cells, some medullary
thymocytes, post-thymic naïve T-lymphocytes, and 80% of B-cell lymphomas. It is a useful marker for distinguishing B-cell lymphomas from
T-cell lymphomas.
Lymphoma
CD45R
CD20
CD43
CD45RO
CD3
Mature B-cell
+
+
-
-
-
Mature T-cell
-
-
+
+
+
Reference
1. Hall PA, et al. J. of Clinical Pathology. 1987; 40:870-873.
2. Myskow MW, et al. American J. of Pathology. 1988; 90:564-574.
3. West KP, et al. J. of Pathology. 1986; 150:89-101.
4. Poppema S, et al. Am. J. of Pathology. 1987; 127:418-429.
5. Norton AJ, et al. Histopathology. 1986; 10:1243-1260.
6. Lauritzen AF, et al. APMIS. 1991 Jul; 99(7):631-9.
7. Sott CS, et al. Clin Exp Immunol. 1991 Dec; 86(3):500-5.
8. Master PS, et al. Int J Hematol. 1992 Jun; 55(3):235-42.
9. Shin SS, et al. Hum Pathol. 1992 Jun; 23(6):686-94.
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