Antibodies for
Immunohistochemistry
CD43 (MT1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45183 IMPATH CD43 RTU M (MT1)
50 Tests
44239 CD43 RTU M (MT1)
7 ml Ready To Use
44500 CD43 0,1 M (MT1)
100 µl liquid Concentrated
44501 CD43 1 M (MT1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Tonsil, Lymph Node
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The literature indicates that from 70% to 90% of T-cell lymphomas and from 22% to 37% of B-cell lymphomas express CD43. No reactivity
has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma,
especially a low grade lymphoma, rather than a reactive B-cell population. When anti-CD43 is used in combination with anti-CD20, effective
immunophenotyping of the lymphomas in paraffin-embedded tissue sections can be obtained. Co-staining of a lymphoid infiltrate with anti-
CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Lymphoma
CD43
CD20
CD45R
CD45RO
CD3
Mature B-cell
-
+
+
-
-
Mature T-cell
+
-
-
+
+
Acute Myeloid Leukemia
CD43
MPO CD68 Factor VIII
CD61 BOB.1 Oct-2 Glyco-
phorin A CD71
CD34
Acute Myeloid, M0
+
-
-
-
-
-
-
-
-
+
Acute Myeloid, M1&2
+
+
+
-
-
-
-
+
Promyelocytic, M3
+
+
-
-
-
+
+
-
-
-
Myelomonocytic, M4
+
+
+
-
-
-
+
-
-
+
Monoblastic, M5
+
+
+
-
-
-
+
-
-
-/+
Acute Erythroid, M6
+/-
+
-
-
-
-
-
+
+
-/+
Megakaryoblastic, M7
+/-
-
-
+
+
+/-
-
-
-
-
Plasma Cells
CD43
CD138
CD79a
EMA
MUM1
CD56 Cyclin D1 CD20
CD19
Plasma Cell Neoplasm
-
+
+
+
+
+
-/+
-/+
-
Reference
1. Cabecades JM, et al. Histopathology. 1991; 19:419-424.
2. Strickler JG, et al. Hum Pathol. 1987; 18:808-814.
3. Sheibani K, et al. Hum Pathol. 1987; 18:1051-1062.
4. Chan JKC, et al. Histopathology. 1988; 12:461-480.
5. Arber DA, et al. App Immunohistochem. 1993; 1:88-96.
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