Antibodies for
Immunohistochemistry
CD45 (LCA) (2B11 & PD7/26)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45185 IMPATH CD45 RTU M (2B11 & PD7/26)
50 Tests
44241 CD45 RTU M (2B11 & PD7/26)
7 ml Ready To Use
44504 CD45 0,1 M (2B11 & PD7/26)
100 µl liquid Concentrated
44577 CD45 1 M (2B11 & PD7/26)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Lymphoma, Tonsil, Lymph Node
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD45 (Anti-Leukocyte Common Antigen) is routinely used to aid the differential diagnosis of undifferentiated neoplasms, whenever malignant
lymphoma is suspected by the morphological or clinical data. It is a highly specific antibody, therefore a positive result is highly indicative of
lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some
leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic
lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
B-cell Lymphomas
CD45
CD20 MUM1 BCL2
BCL6
CD5
CD10
CD23 Cyclin D1 TRAcP
Follicular
+
+
-
+
+
-
+
-
-
-
CLL/SLL
+
+
+
+
-
+
-
+
-
-
Mantle Cell
+
+
-/+
+
-
+
-
-
+
-
Marginal Zone
+
+
+
+
-
-
-
-
-
+/-
Lymphoplasmacytic
+
+
+
+
-
-
-
-
-
-
Diffuse Large Cell
+
+
+
+
+
-/+
-/+
-
-
-
Burkitt
+
+
-
-
+
-
+
-
-
-
Hairy Cell Leukemia
+
+
+
-
-
-
-
+(weak)/-
+
T-cell Lymphomas
CD45
CD2
CD3
CD4
CD5
CD7
CD8
CD25 CD45RO PD-1
Angioimmunoblastic
+
+
+
+
+
+
-
+
+
+
Lymphoblastic
+
+/-
+
+/-
+
+
+/-
+
+
-
Subcutaneous
Panniculitis-Like
+
+
+
-
+
+
+/-
-
+
-
NK
+
+
+
-
-
-/+
-
+
+
-
Cutaneous
+
+
+
+
-
+
-
-
-
-/+
Peripheral, NOS
+
+
+
+/-
+/-
+/-
-/+
+
+
-
Mycosis Fungoides
+
+
+
+
+
-
-
+
+
-
Reference
1. Mason DY. Am J Pathol. 1987; 128:1-4.
2. Hall PA, et al. Histopathology. 1988; 13:149-160.
3. Kurtin PJ, Pinkus GS. Hum Path. 1985; 16:353-365.
4. Maluf HM, et al. Mod Pathol. 1995; 8:155-9.
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