Antibodies for
Immunohistochemistry
GCDFP-15 (EP1582Y
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45285 IMPATH GCDFP-15 RTU R (EP1582Y)
50 Tests
44300 GCDFP-15 RTU R (EP1582Y)
7 ml Ready To Use
44622 GCDFP-15 0,1 R (EP1582Y)
100 µl liquid Concentrated
44623 GCDFP-15 1 R (EP1582Y)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Breast Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
GCDFP-15 is a 15 kD glycoprotein which is localized in the apocrine metaplastic epithelium lining breast cysts and in apocrine glands in the
axilla, vulva, eyelid, and ear canal. Approximately 70% of breast carcinomas stain positive with antibody to GCDFP-15. Anti-GCDFP-15 is the
most specific marker for breast carcinoma. Colorectal carcinomas, lung carcinoma, and mesotheliomas usually do not stain with this antibody.
Lung adenocarcinoma rarely stains with this antibody.
Carcinomas
GCDFP-15 CK 7
CK 20 CK, LMW CK, HMW CK 5
p63 Vimentin ER/PR CEA
Salivary Gland Carcinoma
+
+
-
+
+
+
+
-
+
Breast Carcinoma
+
+
-
+
+
-
-
-
+
-
Sweat Gland Carcinoma
+
+
-
+
+
-
-
+
Carcinoma: Differential Diagnosis
GCDFP-15
Androgen
Receptor
BCA-225
ER/PR Mammaglobin PSA/PSAP
CD44
Salivary Duct Carcinoma
+
+
+
-
-
-
-
Breast Carcinoma
+
+(apocrine)
+
+/-
+
-
-
Prostate Carcinoma
-
+
-
-
-
+
+
Lung Carcinoma
-
+/-
-
-
-
Breast Lesion
GCDFP-15
Mammaglobin
β-Catenin
E-cadherin
CK, 34βE12
p120
Lobular
+
+
-
-
+
+(cytoplasmic)
Ductal
+
+
+(membranous)
+
-
+(membranous)
Breast vs. Lung vs. Prostate Carcinoma
GCDFP-15
Mammaglobin
PSA
TTF-1
Napsin A
Breast Carcinoma
+
+
-
-
-
Lung Carcinoma
-
-
-
+
+
Prostate Carcinoma
-
-
+
-
-
Reference
1. Mazoujian G, Mrgolis R. Am J Dermatopathol. 1988 Feb; 10(1):28-35.
2. Ansai S, et al. Am J Dermatopathol. 1995 Jun; 17(3):249-55.
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