Advanced Solutions
for Advanced Pathology
Glucagon (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45145 IMPATH Glucagon RTU R (Poly)
50 Tests
44295 Glucagon RTU R (Poly)
7 ml Ready To Use
44612 Glucagon 0,1 R (Poly)
100 µl liquid Concentrated
44613 Glucagon 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pancreas
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-glucagon detects glucagon-secreting cells and tumors such as glucagonomas. Studies show that approximately 80% of glucagonomas are
malignant and these patients have a syndrome often initially recognized by dermatologists. Symptoms include necrolytic migratory erythema as
well as diabetes, anemia, stomatitis, weight loss, frequent venous thromboses, and in some instances, diarrhea and psychiatric disturbances.
The diagnosis may be readily confirmed by the demonstration of elevated plasma glucagon concentration.
Pancreas
Glucagon Synapto-
physin
Chromo-
granin A Gastrin CD56 β-Catenin CK 19 CA19-9 E-cadherin CD10
Neuroendocrine Tumor
+/-
+
+
+/-
+
+
+/-
+/-
-
-
Solid Pseudopapillary
Tumor
-
+
-
-
+
+
-
-
+(nuclear)
+
Ductal Carcinoma
-
-
-
-
-
+/-
+
+
+/-
+/-
Acinic Cell Carcinoma
-
-
-
-
-
+
+
-/+
+
+/-
Pancreatoblastoma
-
-
+
-
+
+
-
-
-
-
Normal Pancreas
+
+
+
-
-
+
-
-
-
-
Reference
1. Unger RH, et al. N Eng J Med. 1981; 304:1518-1524.
2. Larson L. Hum Pathol. 1978; 9:401-416.
3. Erlandsen SL. Williams and Wilkins. Baltimore, 1980; 140-155.
4. Friesen SR. N Eng J Med. 1982; 306:580-590.
5. Weitgasser R, et al. Appl Immunohistochem Mol Morphol. 2001 Mar; 9(1):92-6.
6. Bordi C, et al. Am J Clin Pathol. 1987 Aug; 88(2):153-61.
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