Antibodies for
Immunohistochemistry
GLUT1 (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45305 IMPATH GLUT1 RTU R (Poly)
50 Tests
44296 GLUT1 RTU R (Poly)
7 ml Ready To Use
44614 GLUT1 0,1 R (Poly)
100 µl liquid Concentrated
44615 GLUT1 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Colorectal carcinoma, Malignant
mesothelioma
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) or
AP Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Glucose transporter type I (GLUT1), a prototype member of the GLUT superfamily, is a membrane-associated, erythrocyte glucose transport
protein. It is a major glucose transporter in the mammalian blood-brain barrier, and also mediates glucose transport in endothelial cells of the
vasculature, adipose tissue, and cardiac muscle. GLUT1 is detectable in many human tissues including those of colon, lung, stomach, esophagus,
and breast. GLUT1 is overexpressed in malignant cells and in a variety of tumors that include the breast, pancreas, cervix, endometrium,
lung, mesothelium, colon, bladder, thyroid, bone, soft tissues, and oral cavity. Immunohistochemical detection of GLUT1 has been shown to
discriminate between reactive mesothelium and malignant mesothelioma in more than one study. Anti-GLUT1, anti-claudin1, and anti-EMA are
“perineurial” markers that are useful in the diagnosis of perineuriomas. Anti-GLUT1 is also useful in distinguishing benign endometrial hyperplasia
from atypical endometrial hyperplasia and adenocarcinoma. GLUT1 expression has been shown to be associated with increased malignant
potential, invasiveness, and a poor prognosis in general. Expression of GLUT1 is a late event in colorectal cancer and expression in a high
proportion of cancer cells is associated with a high incidence of lymph node metastases.
Mesothelial Cells: Malignant vs. Benign
GLUT1
Mesothelin
Calretinin
p53
Malignant
+
+
+
+
Reactive Benign
-
+
+
-
Skin: Spindle Cell Tumors
GLUT1 SM Actin BG8 Factor VIII Collagen
IV
FLI-1
CD34
CD31 Factor
XIIIa
CD99
Solitary Fibrous Tumor
-
-
-
-
-
-/+
+
-
+/-
+/-
Hemangioma
+
+
+
+
+
+
+
+
-
-
Perineurioma vs. Neurofibroma
GLUT1
Claudin 1
EMA
S-100
Perineurioma
+
+
+
-
Neurofibroma
-
+
+
+
Reference
1. Kato Y, et al. Mod Pathol. 2007 Feb; 20(2):215-20. Epub 2006 Dec 22.
2. Acurio A, et al. Mod Pathol. 2008; 21:334A.
3. Afify A, et al. Acta Cytol. 2005 Nov-Dec; 49(6):621-6.
4. Parente P, et al. Journal of Experimental & Clinical Cancer Research. 2008; 27:34.
5. Zimmerman RL, et al. Cancer. 2002 Feb 25; 96(1):53-7.
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