Advanced Solutions
for Advanced Pathology
Glycophorin A (GA-R2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45306 IMPATH Glycophorin A RTU M (GA-R2)
50 Tests
44297 Glycophorin A RTU M (GA-R2 & HIR2)
7 ml Ready To Use
44616 Glycophorin A 0,1 M (GA-R2 & HIR2)
100 µl liquid Concentrated
44617 Glycophorin A 1 M (GA-R2 & HIR2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Bone Marrow
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Glycophorins A (GPA) and B (GPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for
the MN and SU blood groups. Glycophorins span the membrane and present their amino-terminal end to the extracellular surface of the human
erythrocyte. The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual.
GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large
mucin-like surface and it has been suggested this provides a barrier to cell fusion thus minimizing aggregation between red blood cells in the
circulation. Anti-glycophorin A has been used to characterize erythroid cell development and in the diagnosis of erythroid leukemias.
Acute Myeloid Leukemia
Glyco-
phorin A MPO CD68 Factor VIII
CD61 Lysozyme BOB.1 Oct-2
CD34 CD117
Acute Myeloid, M0
-
-
-
-
-
+
-
-
+
+
Acute Myeloid, M1&2
-
+
+
-
-
+
+
+
Promyelocytic, M3
-
+
-
-
-
-
+
+
-
+
Myelomonocytic, M4
-
+
+
-
-
+
-
+
+
+
Monoblastic, M5
-
+
+
-
-
+
-
+
-/+
+
Acute Erythroid, M6
+
+
-
-
-
-
-
-/+
+/-
Megakaryoblastic, M7
-
-
-
+
+
+/-
-
-
-
Reference
1. van der Valk P, et al. Am J Surg Pathol. 1989 Feb; 13(2):97-106.
2. Muller M, et al. J Bet Med A Physiol Pathol Clin Med. 2001 Feb; 48(1):51-7.
3. Sadahira Y, et al. J Clin Pathol. 1999 Dec; 52(12):919-21.
4. Chang CC, et al. Am J Clin Pathol. 2000 Nov; 114(5):807-11.
5. Muller M, et al. J Vet Med A Physiol Pathol Clin Med. 2001 Feb; 48(1):51-7.
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