Antibodies for
Immunohistochemistry
Glypican-3 (1G12)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45307 IMPATH Glypican-3 RTU M (1G12)
50 Tests
44298 Glypican-3 RTU M (1G12)
7 ml Ready To Use
44618 Glypican-3 0,1 M (1G12)
100 µl liquid Concentrated
44619 Glypican-3 1 M (1G12)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Hepatocellular carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has
been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell
tumors, and Wilms’ tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable
in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic
liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of
testicular germ cell tumors, GPC3 expression is upregulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A
high level of GPC3 expression has also been found in some types of embryonal tumors, such as Wilms’ tumor and hepatoblastoma, with a low
or undetectable expression in normal adjacent tissue.
Liver: Malignant vs. Benign
Glypican-3 Hep-Par1 CD34
p53
AFP
A1AT
pCEA
mCEA
TTF-1
Hepatocellular Carcinoma
+
+
+
+
-/+
-/+
+
-
+
Cytoplasmic
Hepatoblastoma
+
+
-
+
+
+
+
-
-
Benign Liver Nodules
-
+
-
-
-
+/-
-
-
+
Cytoplasmic
Gonads: Germ Cell Tumors vs. Somatic Adenocarcinoma
Glypican-3 Oct-4
AFP Vimentin EMA Inhibin
hPL
CD30 CD117 PLAP
Seminoma
-
+
-
+
-
-
-
-
+
+
Embryonal Carcinoma
-
+
-
-
-
-
-
+
-
+
Choriocarcinoma
+
-
-
-/+
+
-
+
-
-
+
Yolk Sac Tumor
+
-
+
-
-
-
-
-
-
+
Somatic Carcinoma
-
-
-
-
+
-
-
-
-
-
Granulosa Cell Tumor
-
-
-
+
-
+
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
-
+
-
-
-
-
-
Reference
1. Capurro M, et al. Gastroenterology. 2003 Jul; 125(1):89-97.
2. Coston WMP, et al. Am J Surg Pathol. 2008; 00(00):1-12.
3. Kandil D, et al. Cancer. 2007 Oct 25; 111(5):316-22.
4. Kakar S, et al. Arch Pathol Lab Med. 2007 Nov; 131(11):1648-54. Review.
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