Advanced Solutions
for Advanced Pathology
Granzyme B (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45211 IMPATH Granzyme B RTU R (Poly)
50 Tests
44299 Granzyme B RTU R (Poly)
7 ml Ready To Use
44620 Granzyme B 0,1 R (Poly)
100 µl liquid Concentrated
44621 Granzyme B 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Spleen, Anaplastic large cell
lymphoma
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Granzymes are serine proteases which are stored in specialized lytic granules of cytotoxic T-lymphocytes and in natural killer cells. Anti-granzyme
B has been useful in diagnosing natural killer/T-cell lymphoma, as well as anaplastic large cell lymphoma. High percentages of cytotoxic T-cells
have been shown to be an unfavorable prognostic indicator in Hodgkin disease.
T-cell Lymphomas
Granzyme
B
CD2
CD3
CD4
CD5
CD7
CD8
CD25 CD45RO PD-1
Angioimmunoblastic
-
+
+
+
+
+
-
+
+
+
Lymphoblastic
+/-
+/-
+
+/-
+
+
+/-
+
+
-
Subcutaneous
Panniculitis-Like
+
+
+
-
+
+
+/-
-
+
-
NK
+
+
+
-
-
-/+
-
+
+
-
Cutaneous
+
+
+
+
-
+
-
-
-
-/+
Peripheral, NOS
-/+
+
+
+/-
+/-
+/-
-/+
+
+
-
Mycosis Fungoides
+/-
+
+
+
+
-
-
+
+
-
Hodgkin vs. Non-Hodgkin Lymphomas
Granzyme
B
CD79a CD15
CD30 Fascin BCL6
PU.1
MUM1 ALK-1
EMA
Hodgkin Lymphoma,
Classic
-
-
+
+
+
-
-
+
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
-
+
-
-
-
+
+
-/+
-
+
T-cell Rich LBCL
-
+
-
-
-
+
-
+
-
-
Anaplastic Large Cell
Lymphoma
+
-
-
+
-
+/-
-
-
+
+
Reference
1. Oudejans JJ, et al. Blood. 1997 Feb 15; 89(4):1376-82.
2. Oudejans JJ, et al. Am J Pathol. 1996 Jan; 148(1):233-40.
3. Liu J, et al. J Dermatol. 2003 Oct; 30(10):735-41.
4. Kato N, et al. Am J Dermatopathol. 2003 Apr; 25(2):142-7.
5. Kummer JA, et al. Clin Exp Immunol. 1995; 100:164-172.
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