Antibodies for
Immunohistochemistry
Hemoglobin A (EPR3608
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45286 IMPATH HEMOGLOBIN A RTU R (EPR3608)
50 Tests
44303 HEMOGLOBIN A RTU R (EPR3608)
7 ml Ready To Use
44628 HEMOGLOBIN A 0,1 R (EPR3608)
100 µl liquid Concentrated
44629 HEMOGLOBIN A 1 R (EPR3608)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Bone Marrow, Spleen
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Hemoglobin alpha chain belongs to the globin family and is involved in oxygen transport from the lung to the various peripheral tissues.
Hemoglobin A is comprised of two alpha chains and two beta chains, whereas hemoglobin A2 is comprised of two alpha chains and two delta
chains. Immunohistochemical localization of hemoglobin is excellent as an erythroid marker for the detection of immature, dysplastic, and
megaloblastic erythroid cells in myeloproliferative disorders, such as erythroleukemia. In contrast, myeloid cells, lymphoid cells, plasma cells,
histiocytes, and megakaryocytes stain negative with anti-hemoglobin A. Anti-hemoglobin A, combined with antibodies against CD34, CD117,
CD68, and MPO can be helpful in distinguishing between reactive extramedullary hematopoiesis and that seen in neoplastic myeloid disorders
in spleen. Anti-hemoglobin A is limited to expression by erythroid precursors in bone marrow and is therefore of assistance in calculating
percentages of erythroid precursors.
Splenic Hematopoietic Proliferations in Neoplastic and Benign Disorders
Hemoglobin A
MPO
CD34
CD117
CD68
Chronic Myelogenous
Leukemia
-
+
-/+
+/-
+
Chronic Idiopathic
Myelofibrosis
-
+
+/-
-/+
Myelodysplastic Syndrome
-
+
-/+
Myelodysplastic/
Myeloproliferative Disorders
-
+
-
-
+
Mastocytosis
-
+
-
+
Erythroid Disorders
+
+/-
-
-
-/+
Splenic Lymphoma
-
-/+
-
-
Acute Myeloid Leukemia
-
+
+
+
+
Polycythemia Vera
+
+
+
Reference
1. O’Malley DP, et al. Mod Pathol. 2005; 18:550-1561.
2. Dunphy CH, et al. Appl Immun Mol Morphol. 2005; 15(2):154-159.
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