Antibodies for
Immunohistochemistry
hCG (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45148 IMPATH Hcg RTU R (Poly)
50 Tests
44312 hCG RTU R (Poly)
7 ml Ready To Use
44646 hCG (beta) 0,1 R (Poly)
100 µl liquid Concentrated
44647 hCG (beta) 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Placenta
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
hCG is a protein secreted in large quantities by normal trophoblasts; the antibody detects cells and tumors of trophoblastic origin such as
choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively.
20% of lung squamous cell carcinomas are positive for anti-hCG. hCG expression by non-trophoblastic tumors may indicate aggressive behavior
since it has been observed that hCG may play a role in the host response to a given tumor.
Gonads: Germ Cell Tumors vs. Somatic Adenocarcinoma
hCG Oct-4
AFP
EMA Inhibin D2-40
CD30 Glypican-3 CD117 PLAP
Seminoma
-
+
-
-
-
+
-
-
+
+
Embryonal Carcinoma
-
+
-
-
-
-
+
-
-
+
Choriocarcinoma
+
-
-
+
-
-
-
+
-
+
Yolk Sac Tumor
-
-
+
-
-
-
-
+
-
+
Somatic Carcinoma
-
-
-
+
-
-
-
-
-
Granulosa Cell Tumor
-
-
-
-
+
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
+
-
+
-
-
-
-
Placental Trophoblastic Cells
1st Trimester
2nd Trimester
3rd Trimester
hCG
hPL
hCG
hPL
hCG
hPL
Cytotrophoblast
-
-
-
-
-
-
Intermediate Trophoblast
1-24%
25-49%
-/+
50-74%
1-24%
1-49%
Syncytiotrophoblast
>75%
1-24%
25-49%
50-74%
1-24%
>75%
Placental Trophoblastic Proliferations
hCG
p57
PLAP
hPL
CK, OSCAR
Vimentin
Partial Mole
Weak, diffuse
+
+
Weak, diffuse
Strong, diffuse
-
Complete Mole
Strong, diffuse
-
Weak, focal
Weak, focal
Strong, diffuse
-
Choriocarcinoma
Strong, diffuse
-
Weak, focal
Weak, focal
Strong, diffuse
-/+
Placental Site Tumor
Strong, focal
Strong, diffuse Strong, diffuse Strong, diffuse Strong, diffuse
Reference
1. Morrish DW, et al. J Histochem Cytochem. 1987; 35:39-101.
2. Kurman RJ, et al. Cancer. 1976; 38:2404-2419.
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