Advanced Solutions
for Advanced Pathology
Helicobacter pylori (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45146 IMPATH Helicobacter pylori RTU R (Poly)
50 Tests
44302 Helicobacter pylori RTU R (Poly)
7 ml Ready To Use
44626 Helicobacter pylori 0,1 R (Poly)
100 µl liquid Concentrated
44627 Helicobacter pylori 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cell Wall
Control
H. Pylori infected stomach tissue
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development
of gastric malignancy, peptic ulcers, and chronic gastritis in humans. It is associated with the development of adenocarcinoma and low grade
lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of
vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others
do not. The bacteria are most likely spread from person to person through fecal to oral or oral to oral routes. Possible environmental sources
include contaminated water reservoirs. Helicobacter pylori can exist in a number of locations: in the mucus, attached to epithelial cells, or inside
of vacuoles in epithelial cells, where it produces adhesions that bind to membrane-associated lipids and carbohydrates in or on epithelial cells.
One can test noninvasively for H. pylori infection with a blood antibody test, stool antigen test, or with the carbon urea breath test (in which
the patient drinks 14C- or 13C-labelled urea, which the bacterium metabolizes, producing labelled carbon dioxide that can be detected in the
breath). However, the most reliable method for detecting H. pylori infection is a biopsy during endoscopy with a rapid urease test, histologic
examination, and microbial culture. None of the test methods are completely failsafe. Even a biopsy as a test method is dependent on the
location of the biopsy. Blood antibody tests, for example, range from 76% to 84% sensitivity. Some drugs can affect H. pylori urease activity and
give false negatives with the urea-based tests. Immunohistochemistry staining anti-H.pylori on the surface and stomach mucosa is a valuable
tool for diagnosis of H. pylori infections.
Reference
1. Toulaymat M, et al. Arch Pathol Lab Med. 1999 Sep; 123(9):778-81.
2. Cartun RW, et al. Modern Pathology. 1991; Vol. 4, No. 4:498-502.
3. Shimizu T, et al. Helicobacter. 1996 Dec; 1(4):197-206.
4. Jhala NC, et al. Am J Clin Pathol. 2003; 119:101-107.
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