Antibodies for
Immunohistochemistry
Mammaglobin (31A5)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45650 IMPATH Mammaglobin RTU C (304-1A5/31A5) 50 Tests
44331 Mammaglobin RTU R (31A5)
7 ml Ready To Use
44684 Mammaglobin 0,1 R (31A5)
100 µl liquid Concentrated
44685 Mammaglobin 1 R (31A5)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Breast Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Mammaglobin is a 10 kDa glycoprotein with unknown function identified in a substantial proportion of primary and metastatic breast carcinomas.
Mammaglobin mRNA is present in high levels in human breast cancer cell lines and primary breast cancers. High levels of mRNA have been
detected in normal human sweat glands as well, and anti-mammaglobin may label sweat gland tumors. Anti-mammaglobin has been shown to
be effective in detecting up to 85% of breast carcinomas via immunohistochemistry on paraffin-embedded tissues. Anti-mammaglobin is higher
in sensitivity and lower in specificity for primary and metastatic breast carcinoma, in comparison to anti-GCDFP-15.
Carcinoma: Differential Diagnosis
Mammaglobin Androgen
Receptor
BCA-225
GCDFP-15
ER/PR
PSA/PSAP
CD44
Salivary Duct Carcinoma
-
+
+
+
-
-
-
Breast Carcinoma
+
+(apocrine)
+
+
+/-
-
-
Prostate Carcinoma
-
+
-
-
-
+
+
Breast Lesion
Mammaglobin
GCDFP-15
β-Catenin
E-cadherin
CK, 34βE12
p120
Lobular
+
+
-
-
+
+(cytoplasmic)
Ductal
+
+
+(membranous)
+
-
+(membranous)
Breast vs. Lung vs. Prostate Carcinoma
Mammaglobin
GCDFP-15
PSA
TTF-1
Napsin A
Breast Carcinoma
+
+
-
-
-
Lung Carcinoma
-
-
-
+
+
Prostate Carcinoma
-
-
+
-
-
Reference
1. Watson MA, et al. Cancer Research. 1999 July; 59:3028-3031.
2. Fleming TP. Ann N Y Acad Sci. 2000; 923:78-89.
3. Han JH, et al. Arch Pathol Lab Med. 2003 Oct; 127:1330-1334.
4. Bhargava R, et al. Am J Clin Pathol. 2007 Jan; 127(1):103-13.
5. Sasak E, et al. Mod Pathol. 2007 Feb; 20 (2):208-14.
6. Wang Z, et al. Int J Clin Exp Pathol. 2009; 2(4):384-9.
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