Advanced Solutions
for Advanced Pathology
Lambda (Lamb14)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44326 Lambda Light Chain RTU M (Lamb14)
7 ml Ready To Use
44674 Lambda Light Chain 0,1 M (Lamb14)
100 µl liquid Concentrated
44675 Lambda Light Chain 1 M (Lamb14)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Anti-lambda detects surface immunoglobulin on normal and neoplastic B-cells. Anti-lambda staining is seen in B-cell follicles of human lymphoid
tissue. When dealing with B-cell neoplasms, the determination of light chain ratios remains helpful. Most B-cell lymphomas express either kappa
or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is
detected, a lymphoproliferative disorder is very likely. Monoclonality is determined by a kappa-lambda ratio of greater than 3:1, a lambda-kappa
ratio greater than 2:1.
Immunoglobulin, Heavy and Light Chain
Lambda
IgA
IgG
IgD
IgM
Kappa
Myeloma
-/+
+
+
-/+
-/+
+/-
Diffuse LBCL
-/+
-
+
-
+
+/-
Marginal Zone Lymphoma
-/+
-
-
-/+
+
+/-
SLL/CLL
-/+
-
-
+
+
+/-
B-cell Lymphomas
Lambda Kappa CD79a BCL2
BCL6
CD5
CD10
CD23 Cyclin D1 Annexin
A1
Follicular
-/+
+/-
+
+
+
-
+
-
-
-
CLL/SLL
-/+
+/-
+
+
-
+
-
+
-
-
Mantle Cell
-/+
+/-
+
+
-
+
-
-
+
-
Marginal Zone
-/+
+/-
+
+
-
-
-
-
-
-
Lymphoplasmacytic
-/+
+/-
+
+
-
-
-
-
-
-
Diffuse Large Cell
-/+
+/-
+
+
+
-/+
-/+
-
-
-
Burkitt
-/+
+/-
+
-
+
-
+
-
-
-
Hairy Cell Leukemia
-/+
+/-
+
+
-
-
-
-
+(weak)/-
+
Reference
1. Michie SA, et al. A J Clin Path. 1987.
2. Hertel BF, et al. Lab Invest. 1977; 36:12.
3. Taylor CL. Arch Pathol Lab Med. 1978; 12:113-121.
4. Abbondanzo SL, et al. Ann Diagn Pathol. 1999 Oct; 3(6):394.
5. Kurtin PJ, et al. Am J Clin Pathol. 1999 Sep; 112(3):319-29.
6. Ashton-Key M, et al. Histopathology. 1996 Dec; 29(6):525-31.
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