Advanced Solutions
for Advanced Pathology
Ki-67 (EP5
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44324 Ki67 Antigen RTU R (EP5)
7 ml Ready To Use
44670 Ki67 Antigen 0,1 R (EP5)
100 µl liquid Concentrated
44671 Ki67 Antigen 1 R (EP5)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
The Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. In general, Ki-67 is a good marker
of proliferating cell populations. Anti-Ki-67 labeling index has been shown to be a good prognostic marker in a number of neoplasms including
grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma.
Bladder: Dysplasia vs. Reactive
Ki-67
CK 20
p53
CD44
Carcinoma-in-situ
+
+
+
-
Reactive Atypia
+
-
-
+ (all cell layers)
Normal Urothelium
-
+ (umbrella cells)
-
+ (umbrella cells)
Cervix
Ki-67
BCL2
CK 17
Cervical Intraepithelial
Neoplasia
+
-
-
Tubo-Endometrial
Metaplasia
-
+
+
Microglandular Hyperplasia
-
-
-
Reference
1. Mckeever P, et al. J Neuropathol Exp Neurol. 1998; 57:931-936.
2. Coons SW, et al. Neurosurgery. 1997 Oct; 41(4):878-84.
3. Allegra CJ, et al. J Clin Oncol. 2003 Jan 15; 21(2):241-50.
4. Trihia H, et al. Cancer. 2003 Mar 1; 97(5):1321-31.
5. Veronese SM, et al. Anticancer Res. 1995 Nov-Dec; 15(6B):2717-22.
6. Bacus SS, et al. Am J Pathol. 1989; 135:783-792.
7. Gonzalez-Vela MC, et al. Histol Histopathol. 2001 Apr; 16(2):399-406.
8. Imamura H, et al. Jpn J Cancer Res. 1997 Oct; 88(10):1017-23.
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